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Characterization of single-cell electroporation by using patch-clamp and fluorescence microscopy.
Electroporation of single NG108-15 cells with carbon-fiber microelectrodes was characterized by patch-clamp recordings and fluorescence microscopy. To minimize adverse capacitive charging effects,Expand
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Single-cell electroporation.
Electroporation is a widely used method for the introduction of polar and charged agents such as dyes, drugs, DNA, RNA, proteins, peptides, and amino acids into cells. Traditionally, electroporationExpand
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Molecular engineering: Networks of nanotubes and containers
We have constructed complex two-dimensional microscopic networks of phospholipid bilayer nanotubes and containers in which we are able to control the connectivity, container size, nanotube length,Expand
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Electroinjection of colloid particles and biopolymers into single unilamellar liposomes and cells for bioanalytical applications.
A combined electroporation and pressure-driven microinjection method for efficient loading of biopolymers and colloidal particles into single-cell-sized unilamellar liposomes was developed. SingleExpand
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Manipulating the biochemical nanoenvironment around single molecules contained within vesicles
Abstract A method to study single-molecule reactions confined in a biomimetic container is described. The technique combines rapid vesicle preparation, optical trapping and fluorescence confocalExpand
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Manipulating the genetic identity and biochemical surface properties of individual cells with electric-field-induced fusion.
A method for cell-cell and cell-liposome fusion at the single-cell level is described. Individual cells or liposomes were first selected and manipulated either by optical trapping or by adhesion to aExpand
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Chemical transformations in individual ultrasmall biomimetic containers.
Individual phospholipid vesicles, 1 to 5 micrometers in diameter, containing a single reagent or a complete reaction system, were immobilized with an infrared laser optical trap or by adhesion toExpand
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Selective Introduction of Antisense Oligonucleotides into Single Adult CNS Progenitor Cells Using Electroporation Demonstrates the Requirement of STAT3 Activation for CNTF-Induced Gliogenesis
We have developed a novel method in which antisense DNA is selectively electroporated into individual adult neural progenitor cells. By electroporation of antisense oligonucleotides against signalExpand
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Microfluidic device for combinatorial fusion of liposomes and cells.
We describe an electrofusion-based technique for combinatorial synthesis of individual liposomes. A prototype device with containers for liposomes of different compositions and a fusion container wasExpand
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Scanning electroporation of selected areas of adherent cell cultures.
We present a computer-controlled scanning electroporation method. Adherent cells are electroporated using an electrolyte-filled capillary in contact with an electrode. The capillary can be scannedExpand
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