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Walking along the rabies genome: is the large G-L intergenic region a remnant gene?
TLDR
Evidence for the existence of a remnant protein gene in the 423 nucleotide long G-L intergenic region of rabies intergenic regions is presented and is discussed in terms of the evolution of unsegmented negative-strand RNA viruses.
Crystal structures of a template‐independent DNA polymerase: murine terminal deoxynucleotidyltransferase
TLDR
The crystal structure of the catalytic core of murine terminal deoxynucleotidyltransferase (TdT) at 2.35 Å resolution reveals a typical DNA polymerase β‐like fold locked in a closed form, suggesting a common two metal ions mechanism of nucleotidol transfer in these two proteins.
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
TLDR
A procedure is described for the large-scale purification of light (L) and heavy (H) chain mRNAs from plasmacytomas produced in mice by oligo(dT)-cellulose chromatography and either sucrose gradient centrifugation in conditions preventing aggregation or by means of high-resolution preparative gel electrophoresis under non-denaturing conditions.
Completion of the rabies virus genome sequence determination: highly conserved domains among the L (polymerase) proteins of unsegmented negative-strand RNA viruses.
TLDR
The terminal complementarity observed in the rabies genome suggests the conservation of important genomic signals, and provides further evidence for a common ancestry of Rhabdoviridae and Paramyxovir families.
Terminal Deoxynucleotidyl Transferase Indiscriminately Incorporates Ribonucleotides and Deoxyribonucleotides*
TLDR
An essential catalytic aspartate in TdT was identified, which is a first step toward understanding the apparent lack of sugar discrimination by Td T, and reflects an impeded accommodation of ribo- or mixed ribo/deoxyribonucleic acid substrates by T dT.
Single-domain antibodies recognize selectively small oligomeric forms of amyloid beta, prevent Abeta-induced neurotoxicity and inhibit fibril formation.
TLDR
It is confirmed that VHHs may recognize non-conventional epitopes and illustrates their potential for the immunodiagnostic of diseases due to protein accumulation and the generation of novel camelid single-chain binding domains (VHHs) that recognizes specifically low molecular-weight oligomers.
Host Alternation Is Necessary to Maintain the Genome Stability of Rift Valley Fever Virus
TLDR
The results strongly support the view that alternating replication is necessary to maintain the virulence factor carried by the NSs phosphoprotein.
Conferring a template-dependent polymerase activity to terminal deoxynucleotidyltransferase by mutations in the Loop1 region
TLDR
Attempts to transform Tdt into pol μ with the minimal number of mutations in and around Loop1 are described, and the single-point mutant F401A displays a sequence-specific replicative polymerase phenotype that is stable upon Co++ addition.
Cell‐penetrating anti‐GFAP VHH and corresponding fluorescent fusion protein VHH‐GFP spontaneously cross the blood‐brain barrier and specifically recognize astrocytes: application to brain imaging
TLDR
It is demonstrated for the first time that a new class of antibodies can cross the blood‐brain barrier without treatment and specifically recognize astrocytes: application to brain imaging.
Tetrameric and Homodimeric Camelid IgGs Originate from the Same IgH Locus
TLDR
Analysis of cDNA coding for the membrane forms of IgG and IgM present in alpaca peripheral blood B cells are most consistent with the notion that the development of a B cell bearing homodimeric IgG passes through an IgM+ stage, similar to the case for conventional IgG.
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