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The structure of the endoribonuclease XendoU: From small nucleolar RNA processing to severe acute respiratory syndrome coronavirus replication
TLDR
The conserved structural determinants of this site may provide a framework for attempting to design antiviral drugs to interfere with the infectious nidovirus life cycle. Expand
Photoconvertible Fluorescent Protein EosFP: Biophysical Properties and Cell Biology Applications
TLDR
A variety of applications in cellular imaging, developmental biology and automated high-content screening applications are presented, which demonstrate that EosFP is a powerful tool for in vivo monitoring of cellular processes. Expand
Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts
TLDR
This work has modeled the 3D structure of dimeric, full-length LRRK2 by combining domain-based homology models with multiple experimental constraints provided by chemical cross-linking combined with mass spectrometry, negative-stain EM, and small-angle X-ray scattering, and reveals dimeric L RRK2 has a compact overall architecture with a tight, multidomain organization. Expand
Structure of γ-Secretase and Its Trimeric Pre-activation Intermediate by Single-particle Electron Microscopy*
TLDR
The three-dimensional reconstruction of a mature and catalytically active γ-secretase using single-particle cryo-electron microscopy is determined and suggests that the incorporation of PEN-2 might contribute to the maturation of the active site architecture. Expand
Chromophore-protein interactions in the anthozoan green fluorescent protein asFP499.
TLDR
A structural and spectroscopic investigation of the green FP asFP499 from the sea anemone Anemonia sulcata var. Expand
Crystallization and preliminary X-ray diffraction analysis of the red fluorescent protein eqFP611.
TLDR
A novel red fluorescent protein, eqFP611, from the sea anemone Entacmaea quadricolor has been cloned in Escherichia coli and shows the most red-shifted emission and the largest Stokes shift of all non-modified proteins in the green fluorescent protein (GFP) family. Expand
Exploring chromophore--protein interactions in fluorescent protein cmFP512 from Cerianthus membranaceus: X-ray structure analysis and optical spectroscopy.
TLDR
A structural and spectroscopic characterization of the green fluorescent protein cmFP512 from Cerianthus membranaceus, a nonbioluminescent, azooxanthellate cnidarian, which has only approximately 22% sequence identity with Aequorea victoria GFP is presented. Expand
Characterization of the sequences encoding for Xenopus laevis box C/D snoRNP Nop56 protein.
TLDR
It is shown that XNOP56 is a common component of X. laevis box C/D snoRNPs and that it displays the same electrophoretic mobility of p62 protein that was previously characterized as a box C-DsnoRNP component, not essential for snoRNA stability in X. Laevis. Expand
Red fluorescent protein eqFP611 and its genetically engineered dimeric variants.
TLDR
The monomer interfaces in the x-ray structure of eqFP611 are examined to provide a basis for the rational design of monomeric variants and structural manipulations in the A/C interface result in essentially complete loss of fluorescence, suggesting that A-C interfacial interactions play a crucial role in the folding of eq FP611 into its functional form. Expand
Large-scale purification and crystallization of the endoribonuclease XendoU: troubleshooting with His-tagged proteins
TLDR
Recombinant His-tagged XendoU, a eukaryotic endoribonuclease, appeared to aggregate in the presence of divalent cations in order to extract crystals diffracting to 2.2 Å. Expand
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