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Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity.
We show here the identity of Alamar Blue as resazurin. The 'resazurin reduction test' has been used for about 50 years to monitor bacterial and yeast contamination of milk, and also for assessingExpand
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Validation and development of fluorescence two‐dimensional differential gel electrophoresis proteomics technology
Fluorescence two‐dimensional differential gel electrophoresis (2‐D DIGE  * Difference Gel Electrophoresis (DIGE) technology is covered by US patent number 6043025 and foreign equivalents and patentsExpand
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Modulation of notch processing by gamma-secretase inhibitors causes intestinal goblet cell metaplasia and induction of genes known to specify gut secretory lineage differentiation.
It is anticipated that gamma-secretase inhibitors (gamma-Sec-I) that modulate Notch processing will alter differentiation in tissues whose architecture is governed by Notch signaling. To explore thisExpand
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Genomics and proteomics analysis of acetaminophen toxicity in mouse liver.
Overdose of acetaminophen (APAP) causes severe centrilobular hepatic necrosis in humans and experimental animals. Here, to explore its mechanism, we administered APAP at subtoxic (150 mg/kg ip) andExpand
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An integrated metabonomic investigation of acetaminophen toxicity in the mouse using NMR spectroscopy.
An integrated metabonomics study using high-resolution 1H NMR spectroscopy has been applied to investigate the biochemical composition of intact liver tissue (using magic angle spinning), liverExpand
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Imatinib does not induce cardiotoxicity at clinically relevant concentrations in preclinical studies.
Cytotoxic concentrations of imatinib mesylate (10-50 microM) were required to trigger markers of apoptosis and endoplasmic reticulum stress response in neonatal rat ventricular myocytes andExpand
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Reduced Activity of Sphingosine-1-Phosphate Lyase Induces Podocyte-related Glomerular Proteinuria, Skin Irritation, and Platelet Activation
Sphingosine-1-phosphate (S1P) lyase is considered as a drug target in autoimmune diseases based on the protective effect of reducing activity of the enzyme in animal models of inflammation. Since S1PExpand
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Integrated application of transcriptomics and metabonomics yields new insight into the toxicity due to paracetamol in the mouse.
Gene chip array (Affymetrix) data from liver tissue and high resolution 1H NMR spectra from intact liver tissue, tissue extracts and plasma have been analyzed to identify biochemical changes arisingExpand
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Perturbation of microRNAs in Rat Heart during Chronic Doxorubicin Treatment
Anti-cancer therapy based on anthracyclines (DNA intercalating Topoisomerase II inhibitors) is limited by adverse effects of these compounds on the cardiovascular system, ultimately causing heartExpand
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Inroads to Predict in Vivo Toxicology—An Introduction to the eTOX Project
There is a widespread awareness that the wealth of preclinical toxicity data that the pharmaceutical industry has generated in recent decades is not exploited as efficiently as it could be. EnhancedExpand
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