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Cloning and nucleotide sequence of a cDNA encoding the precursor of the barley toxin a-hordothionin
- Fernando Ponz Ascaso, Javier Paz Ares, C. Lucas, F. Olmedo, Pilar Carbonero Zalduegui
- 1 April 1986
The deducedThionin sequence is preceded by a leader sequence of 18 residues and followed by a sequence that corresponds to an acidic protein of 64 amino acids, which supports previous evidence indicating that thionin is synthesized as a much larger precursor, which undergoes two processing steps: the cotranslational cleavage of aleader sequence and the post-translational one of a larger peptide.
Inactivation of the sapA to sapF locus of Erwinia chrysanthemi reveals common features in plant and animal bacterial pathogens
Results indicate that the interaction of antimicrobial peptides from the host with the sapA to sapF operon from the pathogen plays a similar role in animal and in plant bacterial pathogenesis.
A multigene family of trypsin/a-amylase inhibitors from cereals
In wheat and barley, a substantial fraction of the total endosperm protein content is represented by toxins and inhibitors that are active towards heterologous systems, and some of them can be selectively extracted with chloroform/methanol mixtures and have therefore been designated CM-proteins.
Continuous production of 5′-ribonucleotides from yeast RNA by hydrolysis with immobilized 5′-phosphodiesterase and 5′-adenylate deaminase
- F. Olmedo, F. Iturbe, J. Gómez-Hernández, A. López-Munguía
- BiologyWorld journal of microbiology & biotechnology
The production of 5-IMP and 5-GMP by enzymatic conversion from RNA using a continuous two packed-bed reactor was investigated, finding no significant differences in the Km value of the free and immobilized 5-Phosphodiesterase and 5′-adenylate deaminase.
Einstein y Picasso
- F. Olmedo
- 1 February 2007
En el pasado siglo no han sido pocos los jovenes que, despues de completar una tesis en fisica teorica, han salido huyendo en otras direcciones, apabullados quizas por las contribuciones precedentes…
The thionins: a protein family that includes purothionins, viscotoxins and crambins
Almost half a century ago, a crystalline protein material was obtained from lipid extracts of wheat endosperm and designated "purothionin" (Trvpwo, wheat); this material was found to have baetericidal and fungicidal properties.
Biochemical evidence of gene transfer from the Mv genome of Aegilops ventricosa to hexaploid wheat
Endosperm protein and isozyme patterns of Unes derived from the ovoss and the wide distribution of one of the markers suggests its previous transfer to the D genome of Ae.
Chromosomal locations of genes that control wheat endosperm proteins
Progress in aneuploid analysis of genes controlling proteins has generally depended on advances in protein fractionation and characterization, but in many cases, these genetic analyses have been done on insufficiently characteri/ed groups of proteins, and this has resulted in some confusion.
Transfer of resistance to eyespot disease from Aegilops ventricosa to wheat
The wild grass Aegilops ventricosa has been recognised for almost 30 years as an important potential donor of genes that determine characters of agronomic interest, which has led to the recent discovery of a major dominant gene for resistance, which confers a high level of resistance to cultivated wheat.
Plant defence peptides
Eight families of antimicrobial peptides, ranging in size from 2 to 9 kD, have been identified in plants, and transgenic overe.xpression of the corresponding genes leads to enhanced tolerance to pathogens, and peptide-sensitive pathogen mutants have reduced virulence.