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Post-mitotic human dermal fibroblasts efficiently support the growth of human follicular keratinocytes.
For growth at low seeding densities, keratinocytes isolated from human tissues like epidermis or hair follicles are dependent on mesenchyme-derived feeder cells such as the 3T3-cell employed so far.Expand
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Serial cultivation of single keratinocytes from the outer root sheath of human scalp hair follicles.
  • A. Limat, F. Noser
  • Biology, Medicine
  • The Journal of investigative dermatology
  • 1 October 1986
A method for the isolation of outer root sheath keratinocytes from plucked human hair follicles and for their subsequent cultivation has been developed. The selective trypsinization of outer rootExpand
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Restoration of the epidermal phenotype by follicular outer root sheath cells in recombinant culture with dermal fibroblasts.
In order to better understand how outer root sheath (ORS) cells are able to reepithelialize superficial skin wounds, the level of epidermal differentiation achieved by isolated ORS cells in vitro wasExpand
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Experimental Modulation of the Differentiated Phenotype of Keratinocytes from Epidermis and Hair Follicle Outer Root Sheath and Matrix Cells
Follicles of human anagen hair were separated into morphologically distinct compartments (by sequential trypsinization and microdissection) for the biochemical and immunological analysis of keratinsExpand
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Studies on the potential in vivo induction of sister-chromatid exchanges in rat bone marrow by resorcinol.
The hair-dye component resorcinol was tested for potential in vivo induction of sister-chromatid exchanges (SCE) in bone-marrow cells after intraperitoneal, peroral and epicutaneous application toExpand
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Outer root sheath (ORS) cells organize into epidermoid cyst-like spheroids when cultured inside Matrigel: a light-microscopic and immunohistological comparison between human ORS cells and
In organotypic cultures, outer root sheath (ORS) cells of the human hair follicle develop into a stratified epithelium largely reminiscent of the epidermis; this apparently reflects their importanceExpand
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Organotypic culture of outer root sheath cells from human hair follicles using a new culture device
  • F. Noser, A. Limat
  • Biology, Medicine
  • In Vitro Cellular & Developmental Biology
  • 1 August 1987
SummaryA new culture vessel for the growth of cells on biological substrata and under organotypic conditions is described. This device, named Combi-ring-dish (CRD), is composed of four concentricExpand
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Differentiation and polypeptide expression in human papilla and dermal fibroblasts in vitro.
Proliferation and differentiation of human scalp hair follicle papilla fibroblasts (PF) in the anagenic stage of development and of neighboring dermal fibroblasts (DF) of the same donor were studiedExpand
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Postmitotic human dermal fibroblasts preserve intact feeder properties for epithelial cell growth after long-term cryopreservation
SummaryIn vitro, human dermal fibroblasts (HDF) differentiate through morphologically and biochemically identified compartments. In the course of this spontaneous differentiation through mitotic andExpand
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Phenotypic modulation of human hair matrix cells (trichocytes) by environmental influence in vitro and in vivo.
Trichocytes, i.e. precursor cells of the hair cortex and medulla, isolated from plucked human scalp hair follicles (HF) were propagated on feeder layers of post-mitotic human dermal fibroblastsExpand
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