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Effect of monosaccharides and disaccharides in Tris-based diluents on motility, acrosome integrity and fertility of pellet frozen ram spermatozoa.
Abstract In semen freezing diluents, sugars may be incorporated as metabolites, as cryoprotectants or to maintain diluent osmolality. This study examined the cryoprotective effects of mono- andExpand
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Urinary corticosterone metabolite responses to capture, and annual patterns of urinary corticosterone in wild and captive endangered Fijian ground frogs (Platymantis vitiana)
This study was based on the development of a non-invasive glucocorticoid enzyme-immunoassay for the assessment of stress in wild and captive endangered Fijian ground frogs (Platymantis vitiana).Expand
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In vitro evaluation of zwitterion buffers in diluents for freezing ram spermatozoa.
Diluents based on the zwitterion buffers Tes, Hepes and Pipes titrated to pH 7.0 with NaOH or Tris were compared with Tris-citrate diluents by assessment of post-thawing motility and acrosomeExpand
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Urinary corticosterone responses to capture and toe-clipping in the cane toad (Rhinella marina) indicate that toe-clipping is a stressor for amphibians.
Toe-clipping, the removal of one or more toes, is a common method used to individually mark free-living animals. Whilst this method is widely used in studies of amphibians, the appropriateness of theExpand
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Emerging trends for biobanking amphibian genetic resources: The hope, reality and challenges for the next decade
How to conserve our planet’s rapidly disappearing biodiversity is one of the greatest challenges of our generation. Among terrestrial vertebrate taxa, amphibians are most at risk with 41% of allExpand
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Individual variation and repeatability in urinary corticosterone metabolite responses to capture in the cane toad (Rhinella marina).
Urinary corticosterone metabolite enzyme-immunoassay (EIA) can be used for the non-invasive assessment of baseline levels and corticosterone responses in amphibians. In this study, urinaryExpand
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Incorporation of penetrating cryoprotectants in diluents for pellet-freezing ram spermatozoa.
Glycerol may be toxic to frozen-thawed ram spermatozoa and reduce their fertilizing capacity. This study examined the cryoprotective effects of dimethyl sulphoxide (DMSO), ethylene glycol, glycerolExpand
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Successful fertilization after superovulation and laparoscopic intrauterine insemination of the brushtail possum, Trichosurus vulpecula, and tammar wallaby, Macropus eugenii.
Fertilization has been achieved in superovulated brushtail possums and tammar wallabies after laparoscopic intrauterine artificial insemination. Various superovulation protocols and inseminationExpand
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Changes in urinary testosterone and corticosterone metabolites during short-term confinement with repeated handling in wild male cane toads (Rhinella marina)
Stressors generally decrease testosterone secretion and inhibit reproduction in animals. Urinary testosterone and corticosterone metabolite concentrations were measured in adult male cane toadsExpand
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Urinary hormone analysis assists reproductive monitoring and sex identification of bell frogs (Litoria raniformis).
With the world currently facing a global amphibian extinction crisis, the development of techniques to help meet the needs of conservation managers and researchers studying the reproductive biologyExpand
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