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The genome sequence of Mycoplasma hyopneumoniae strain 232, the agent of swine mycoplasmosis.
We present the complete genome sequence of Mycoplasma hyopneumoniae, an important member of the porcine respiratory disease complex. The genome is composed of 892,758 bp and has an average G+CExpand
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  • Open Access
Construction of Tn4001lac derivatives to be used as promoter probe vectors in mycoplasmas.
Studies of gene expression in mycoplasmas has been difficult, because the elements involved in gene expression remain relatively undefined. In order to be able to examine these regulatory elements inExpand
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Use of a Mycoplasma Hyopneumoniae Nested Polymerase Chain Reaction Test to Determine the Optimal Sampling Sites in Swine
A number of polymerase chain reaction (PCR)-based diagnostic tests have been developed for Mycoplasma hyopneumoniae, including one from this research group. This report presents further development,Expand
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P159 is a proteolytically processed, surface adhesin of Mycoplasma hyopneumoniae: defined domains of P159 bind heparin and promote adherence to eukaryote cells
Mycoplasma hyopneumoniae, the causative agent of porcine enzootic pneumonia, colonizes the respiratory cilia of affected swine causing significant economic losses to swine production worldwide.Expand
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Cloning and functional analysis of the P97 swine cilium adhesin gene of Mycoplasma hyopneumoniae.
Colonization of the swine respiratory tract by Mycoplasma hyopneumoniae is accomplished by specific binding to the cilia of the mucosal epithelial cells. Previous studies have implicated a 97-kDaExpand
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Proteolytic Processing of the Mycoplasma hyopneumoniae Cilium Adhesin
ABSTRACT Mycoplasma hyopneumoniae is an economically significant swine pathogen that colonizes the respiratory ciliated epithelial cells. Cilium adherence is mediated by P97, a surface proteinExpand
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Real-Time PCR Assays To Address Genetic Diversity among Strains of Mycoplasma hyopneumoniae
ABSTRACT Mycoplasma hyopneumoniae is an important cause of pneumonia in pigs around the world, but confirming its presence in (or absence from) pigs can be difficult. Culture for diagnosis isExpand
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  • Open Access
Identification of the Cilium Binding Epitope of theMycoplasma hyopneumoniae P97 Adhesin
ABSTRACT Mycoplasma hyopneumoniae colonizes the swine respiratory tract at the level of ciliated cells by attaching specifically to the cilium membrane. This interaction involves an adhesin calledExpand
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Membrane-associated nuclease activities in mycoplasmas.
Membrane-associated nucleases of various mycoplasmal species were investigated by using two nuclease assays. A lambda DNA assay was developed to measure nuclease activity associated with whole-cellExpand
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  • Open Access
Transcriptional Profiling of Mycoplasma hyopneumoniae during Heat Shock Using Microarrays
ABSTRACT Bacterial pathogens undergo stress during host colonization and disease processes. These stresses result in changes in gene expression to compensate for potentially lethal environmentsExpand
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  • Open Access