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A role for Rac in Tiaml-induced membrane ruffling and invasion
RHO-LIKE GTPases have been implicated in the regulation of the actin cytoskeleton which controls the morphology, adhesion and motility of cells1-3. Like Ras proteins, they become activated when boundExpand
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Mice deficient in the Rac activator Tiam1 are resistant to Ras-induced skin tumours
Proteins of the Rho family control signalling pathways that regulate the actin cytoskeleton and gene transcription. In vitro studies have implicated Rho-like GTP-hydrolysing enzymes (GTPases) in cellExpand
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A 14 bp promoter element directs the testis specificity of the Drosophila beta 2 tubulin gene.
To analyze the regulation of gene expression during male germ cell development, we investigated the testis‐specific expression of the Drosophila beta 2 tubulin gene. Germ line transformationExpand
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Characterization of a gram-positive broad-host-range plasmid isolated from Lactobacillus hilgardii.
Two plasmids, pLAB1000 and pLAB2000 (3.3 and 9.1 kb, respectively), have been isolated from a grass silage strain of Lactobacillus hilgardii. Both plasmids were cloned in Escherichia coli andExpand
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Integration and expression of alpha-amylase and endoglucanase genes in the Lactobacillus plantarum chromosome.
A commercial grass silage starter strain of Lactobacillus plantarum was transformed by high-frequency electroporation with plasmids containing an alpha-amylase gene from Bacillus stearothermophilusExpand
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Lactobacillus hilgardii plasmid pLAB1000 consists of two functional cassettes commonly found in other gram-positive organisms.
A Lactobacillus hilgardii plasmid, pLAB1000, was studied to understand the organization of autonomous replicons from lactobacilli. Two cassettes could be identified. First, the replication regionExpand
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Arrayed adenoviral expression libraries for functional screening
With the publication of the sequence of the human genome, we are challenged to identify the functions of an estimated 70,000 human genes and the much larger number of proteins encoded by these genes.Expand
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Derivation of clones close to met by preparative field inversion gel electrophoresis.
The molecular analysis of genes identified by mutations is a major problem in mammalian genetics. As a step toward this goal, preparative field inversion gel electrophoresis (FIGE) was used toExpand
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Gene activation and re‐expression of a Trypanosoma brucei variant surface glycoprotein.
The expression of the Trypanosoma brucei variant surface glycoprotein AnTat 1.1 proceeds by a mechanism that transfers a duplicated gene copy into a new genomic environment, the so‐called expressionExpand
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Testis-specific β2 tubulins are identical in Drosophila melanogaster and D. hydei but differ from the ubiquitous β1 tubulin
In Drosophila as in many organisms β tubulins are encoded by a gene family. We have determined the complete nucleotide sequences coding for the β1 and β2 tubulins of Drosophila melanogaster and theExpand
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