After endocytosis, most membrane proteins and lipids return to the cell surface, but some membrane components are delivered to late endosomes or the Golgi, where they are sorted and recycled.
Role of cholesterol and lipid organization in disease
Significant recent progress has enhanced the understanding of the molecular and cellular basis of lipid-associated disorders such as Tangier disease, Niemann–Pick disease type C and atherosclerosis.
Rme-1 regulates the distribution and function of the endocytic recycling compartment in mammalian cells
It is shown that expression of a dominant-negative construct with a point mutation near the EH domain results in redistribution of the endocytic recycling compartment and slowing down of transferrin receptor recycling.
Membrane transport in the endocytic pathway.
Sorting of membrane components from endosomes and subsequent recycling to the cell surface occurs by a bulk flow process
Using quantitative fluorescence microscopy, the endocytic route and kinetics of traffic of the bulk carrier, membrane lipids, are determined and it is shown that N-[N-(7-nitro-2,1,3-benzoxadiazol-4-yl)-epsilon-aminohexanoyl]- sphingosylphosphorylcholine (C6-NBD-SM) in endocytosed as bulk membrane.
Segregation of transferrin to a mildly acidic (pH 6.5) para-golgi compartment in the recycling pathway
An Endocytosed TGN38 Chimeric Protein Is Delivered to the TGN after Trafficking through the Endocytic Recycling Compartment in CHO Cells
- R. Ghosh, W. G. Mallet, T. T. Soe, T. McGraw, F. Maxfield
- BiologyJournal of Cell Biology
- 24 August 1998
To examine TGN38 trafficking from the cell surface to the TGN, CHO cells were stably transfected with a chimeric transmembrane protein, TacTGN38. We used fluorescent and 125I-labeled anti-Tac IgG and…
Sequestration of GPI-anchored proteins in caveolae triggered by cross-linking.
Analysis of the cell surface distribution of the GPI-anchored folate receptor by electron microscopy confirms that multimerization regulates their sequestration in caveolae, but in the absence of agents that promote clustering they are diffusely distributed over the plasma membrane.
Chimeric Forms of Furin and Tgn38 Are Transported from the Plasma Membrane to the Trans-Golgi Network via Distinct Endosomal Pathways
These studies demonstrate the existence of two independent pathways for endosomal transport of proteins to the TGN from the plasma membrane, which are blocked by nocodazole, and the two pathways are also differentially affected by wortmannin.
Dansylcadaverine inhibits internalization of 125I-epidermal growth factor in BALB 3T3 cells.
- H. Haigler, F. Maxfield, M. Willingham, I. Pastan
- BiologyJournal of Biological Chemistry
- 25 February 1980
It is proposed that dansylcadaverine inhibits EGF internalization by preventing it from clustering in clathrin-coated pits.