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Activation of Rad53 kinase in response to DNA damage and its effect in modulating phosphorylation of the lagging strand DNA polymerase
The Saccharomyces cerevisiae Rad53 protein kinase is required for the execution of checkpoint arrest at multiple stages of the cell cycle. We found that Rad53 autophosphorylation activity depends onExpand
Spk1/Rad53 is regulated by Mec1-dependent protein phosphorylation in DNA replication and damage checkpoint pathways.
TLDR
Data support the model that Spk1p is an essential intermediate component in a signal transduction pathway coupling damage and checkpoint functions to cell cycle arrest, mediated through a protein kinase cascade that potentially includes Mec1p and Tel1p as the upstream kinases. Expand
The B subunit of the DNA polymerase alpha-primase complex in Saccharomyces cerevisiae executes an essential function at the initial stage of DNA replication.
TLDR
The characterization of the temperature-sensitive pol12-T9 mutant allele demonstrates that the B subunit is required for in vivo DNA synthesis and correct progression through S phase, and reciprocal shift experiments indicate that the POL12 gene product plays an essential role at the early stage of chromosomal DNA replication, before the hydroxyurea-sensitive step. Expand
Functional Interplay between the 53BP1-Ortholog Rad9 and the Mre11 Complex Regulates Resection, End-Tethering and Repair of a Double-Strand Break
TLDR
Deletion of the yeast 53BP1-ortholog RAD9 reduces Mre11 binding to a DSB, leading to Rad52 recruitment and efficient DSB end-tethering, through an Sgs1-dependent mechanism, and restores DSB repair either in absence of Sae2 or in presence of a nuclease defective MRX complex. Expand
Overlapping mechanisms promote postsynaptic RAD-51 filament disassembly during meiotic double-strand break repair.
TLDR
Results indicate that two compensating activities are required to promote postsynaptic RAD-51 filament disassembly, which are collectively essential for completion of meiotic DSB repair. Expand
POLQ (Pol theta), a DNA polymerase and DNA-dependent ATPase in human cells.
TLDR
The isolation of the full-length human DNA POLQ gene, and an initial characterization of its gene product, DNA polymerase theta, are reported, which showed DNA polymerases activity on nicked double-stranded DNA and on a singly primed DNA template. Expand
DNA nucleotide excision repair-dependent signaling to checkpoint activation
TLDR
It is shown here that in G0/G1 and G2/M phases of the cell cycle, triggering of the DNA damage cascade requires recognition and processing of the lesions by the GG-NER, and loss of TC-NER does not affect checkpoint activation. Expand
Human exonuclease 1 connects nucleotide excision repair (NER) processing with checkpoint activation in response to UV irradiation
TLDR
A key role is revealed for hEXO1 in the UV-induced DNA damage response linking NER to checkpoint activation in human cells, which reduces unscheduled DNA synthesis after UV irradiation and impairs activation of the checkpoint signal transduction cascade in response to UV damage. Expand
POLQ (Pol θ), a DNA polymerase and DNA‐dependent ATPase in human cells
TLDR
The isolation of the full-length human DNA POLQ gene, and an initial characterization of its gene product, DNA polymerase theta, are reported, which showed DNA polymerases activity on nicked double-stranded DNA and on a singly primed DNA template. Expand
A Human DNA Helicase Homologous to the DNA Cross-link Sensitivity Protein Mus308*
  • F. Marini, R. Wood
  • Medicine, Biology
  • The Journal of Biological Chemistry
  • 8 March 2002
TLDR
HEL308 is a single-stranded DNA-dependent ATPase and DNA helicase that readily displaces 20- to 40-mer duplex oligonucleotides and translocates on DNA with 3′ to 5′ polarity and behaves as a multimer upon gel filtration. Expand
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