Organic compounds from diesel exhaust particles elicit a proinflammatory response in human airway epithelial cells and induce cytochrome p450 1A1 expression.
- V. Bonvallot, A. Baeza-Squiban, F. Marano
- BiologyAmerican Journal of Respiratory Cell and…
- 1 October 2001
It is demonstrated, for the first time, in airway epithelial cells in vitro that nDEP induce the expression of the CYP1A1, a cytochrome P450 specifically involved in polycyclic aromatic hydrocarbons metabolism, thereby demonstrating the critical role of organic compounds in the DEP-induced proinflammatory response.
Involvement of reactive oxygen species in the metabolic pathways triggered by diesel exhaust particles in human airway epithelial cells.
- A. Baulig, M. Garlatti, A. Baeza-Squiban
- Biology, ChemistryAmerican Journal of Physiology - Lung cellular…
- 1 September 2003
Diesel exhaust particles generate an oxidative stress, activate the Nrf2 transcription factor, and increase the expression of genes for phase I and II metabolization enzymes, suggesting that DEP-adsorbed organic compounds become bioavailable, activate transcription, and are metabolized since the CYP1A1 enzymatic activity is increased.
Diesel exhaust particles are taken up by human airway epithelial cells in vitro and alter cytokine production.
- S. Boland, A. Baeza-Squiban, F. Marano
- Biology, MedicineAmerican Journal of Physiology - Lung cellular…
- 1 April 1999
The results suggest that DEPs could 1) be phagocytosed by airway epithelial cells and 2) induce a specific inflammatory response.
Oxidative stress and proinflammatory effects of carbon black and titanium dioxide nanoparticles: role of particle surface area and internalized amount.
- Salik Hussain, S. Boland, F. Marano
- BiologyToxicology
- 16 June 2009
Evaluating the toxicity of airborne particulate matter and nanoparticles by measuring oxidative stress potential--a workshop report and consensus statement.
- J. Ayres, P. Borm, J. Froines
- MedicineInhalation Toxicology
- 2008
In vitro cell challenges were preferable to in vitro acellular systems but both have a potential major role to play and offer large cost advantages relative to human or animal inhalation studies and animal in vivo installation experiments.
Evaluating the Toxicity of Airborne Particulate Matter and Nanoparticles by Measuring Oxidative Stress Potential—A Workshop Report and Consensus Statement
- J. Ayres, P. Borm, J. Froines
- Medicine
- 1 January 2008
In vitro test methods have an important role to play in the screening of toxicity in airborne particulate matter and nanoparticles and offer large cost advantages relative to human or animal inhalation studies and animal in vivo installation experiments.
IL-13 alters mucociliary differentiation and ciliary beating of human respiratory epithelial cells.
- J. Laoukili, E. Perret, F. Tournier
- Biology, MedicineJournal of Clinical Investigation
- 15 December 2001
The results suggest that, in human allergic asthmatic responses, IL-13 affects both ciliated and secretory cell differentiation, leading to airway damage and obstruction.
Differential expression and cellular distribution of centrin isoforms during human ciliated cell differentiation in vitro.
- J. Laoukili, E. Perret, F. Tournier
- BiologyJournal of Cell Science
- 15 April 2000
A comparative biochemical and functional analysis of centrin isoforms is performed using a primary culture of human nasal epithelial cells which provides an efficient way to obtain a complete ciliated cell differentiation process.
Mechanisms of GM-CSF increase by diesel exhaust particles in human airway epithelial cells.
- S. Boland, V. Bonvallot, T. Fournier, A. Baeza-Squiban, M. Aubier, F. Marano
- BiologyAmerican Journal of Physiology - Lung cellular…
- 2000
Comparison of the effects of DEP, extracted DEPs, or extracts of DEPs has shown that the increase in GM-CSF release is mainly due to the adsorbed organic compounds and not to the metals present on the DEP surface because the metal chelator desferrioxamine had no inhibitory effect.
Carbon black and titanium dioxide nanoparticles elicit distinct apoptotic pathways in bronchial epithelial cells
- Salik Hussain, L. Thomassen, S. Boland
- BiologyParticle and Fibre Toxicology
- 16 April 2010
Both CB and TiO2 NPs induce apoptotic cell death in bronchial epithelial cells although the final outcome is similar (apoptosis), the molecular pathways activated by NPs differ depending upon the chemical nature of the NPs.
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