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Characterization of glycoproteins and their associated oligosaccharides through the use of endoglycosidases.
- F. Maley, R. B. Trimble, A. Tarentino, T. Plummer
- Chemistry, Medicine
- Analytical biochemistry
- 1 August 1989
Sont passees en revue les differentes endoglycosidases d'origine bacterienne, animale ou humaine, permettant de par leur specificite d'hydrolyse, de differencier les residus glycosyles portes par les… Expand
Immunohistochemical quantitation of thymidylate synthase expression in colorectal cancer metastases predicts for clinical outcome to fluorouracil-based chemotherapy.
- C. Aschele, D. Debernardis, +6 authors A. Sobrero
- Journal of clinical oncology : official journal…
- 1 June 1999
Investigating whether immunohistochemical thymidylate synthase (TS) quantitation predicts for clinical outcome in patients with advanced colorectal cancer treated by fluorouracil (FUra)-based chemotherapy found a significant correlation was found between intratumoral TS expression and all the parameters of clinical outcome analyzed. Expand
Studies on Identifying the Catalytic Role of Glu-204 in the Active Site of Yeast Invertase*
A mechanism is proposed for the hydrolysis of sucrose which involves Asp-23 as a nucleophile and Glu-204 as an acid/base catalyst as well as other residues involved in the catalytic process. Expand
Purification and properties of an endo-beta-N-acetylglucosaminidase from Streptomyces griseus.
An enzyme that hydrolyzes di-N-acetylchitobiose linkages in oligosaccharides and glycoproteins was purified to homogeneity from cultural filtrates of Streptomyces griseus and appears to consist of a single polypeptide chain. Expand
Structure of oligosaccharides on Saccharomyces SUC2 invertase secreted by the methylotrophic yeast Pichia pastoris.
- R. B. Trimble, P. H. Atkinson, J. Tschopp, R. Townsend, F. Maley
- Chemistry, Medicine
- The Journal of biological chemistry
- 5 December 1991
Oligosaccharide pools were analyzed for homogeneity by high-pH anion-exchange chromatography, and structures were assigned using 500 MHz one- and two-dimensional 1H NMR spectroscopy to find isometric configurations unique to lower eukaryotes. Expand
Crystal structure of a deletion mutant of human thymidylate synthase Δ (7–29) and its ternary complex with Tomudex and dUMP
- R. Almog, C. Waddling, F. Maley, G. Maley, P. Van Roey
- Chemistry, Medicine
- Protein science : a publication of the Protein…
- 1 May 2001
Results suggest that formation of a covalent bond between the catalytic cysteine and the substrate dUMP is not required for effective inhibition of human TS by cofactor analogs and could have implications for drug design by eliminating this as a condition for lead compounds. Expand
Structure, multiple site binding, and segmental accommodation in thymidylate synthase on binding dUMP and an anti-folate.
The structure of Escherichia coli thymidylate synthase (TS) complexed with the substrate dUMP and an analogue of the cofactor methylenetetrahydrofolate was solved by multiple isomorphous replacement… Expand
Subunit structure of external invertase from Saccharomyces cerevisiae.
It has been possible to show that carbohydrate-free invertase is composed of two 60,000-dalton subunits, and terminal sequence analysis with carboxypeptidases A, B, and Y provided strong evidence that the subunits are identical. Expand
Overcoming inclusion body formation in a high-level expression system.
- J. Moore, A. Uppal, F. Maley, G. Maley
- Biology, Medicine
- Protein expression and purification
- 1 April 1993
By employing an enriched growth medium during the course of protein induction it was found that the deaminase could be induced in a soluble active form to at least 20% of this organism's cellular protein. Expand
Probing the Folate-binding Site of Human Thymidylate Synthase by Site-directed Mutagenesis
- Y. Tong, X. Liu-Chen, +4 authors J. Bertino
- Medicine, Biology
- The Journal of Biological Chemistry
- 20 November 1998
The human TS mutants (I108A and F225W) confirm the importance of amino acid residues Ile-108 and Phe-225 in the binding of folate and its analogues and may be useful for gene transfer experiments to protect hematopoietic progenitor cells from the toxic effects of these drugs. Expand