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Sister-chromatid separation at anaphase onset is promoted by cleavage of the cohesin subunit Scc1
Cohesion between sister chromatids is established during DNA replication and depends on a multiprotein complex called cohesin. Attachment of sister kinetochores to the mitotic spindle during mitosisExpand
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Molecular cloning and expression of brain-derived neurotrophic factor
DURING the development of the vertebrate nervous system, many neurons depend for survival on interactions with their target cells1. Specific proteins are thought to be released by the target cellsExpand
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A major transmembrane protein of Golgi-derived COPI-coated vesicles involved in coatomer binding
Formation of non-clathrin-coated vesicles requires the recruitment of several cytosolic factors to the Golgi membrane. To identify membrane proteins involved in this budding process, a highlyExpand
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Identification of in vivo substrates of the chaperonin GroEL
The chaperonin GroEL has an essential role in mediating protein folding in the cytosol of Escherichia coli. Here we show that GroEL interacts strongly with a well-defined set of approximately 300Expand
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Tim9, a new component of the TIM22·54 translocase in mitochondria
We have identified Tim9, a new component of the TIM22·54 import machinery, which mediates transport of proteins into the inner membrane of mitochondria. Tim9, an essential protein of SaccharomycesExpand
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A mechanism for repression of class II gene transcription through specific binding of NC2 to TBP‐promoter complexes via heterodimeric histone fold domains.
Negative co‐factor 2 (NC2) regulates transcription of the class II genes through binding to TFIID and inhibition of pre‐initiation complex formation. We have isolated and cloned NC2, and investigatedExpand
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A multiprotein complex mediates the ATP-dependent assembly of spliceosomal U snRNPs
The spliceosomal snRNPs U1, U2, U4 and U5 contain a common RNP structure termed the Sm core that is formed by the binding of Sm proteins onto the U snRNA. Although isolated Sm proteins assembleExpand
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Matching of oligoclonal immunoglobulin transcriptomes and proteomes of cerebrospinal fluid in multiple sclerosis
We describe a method for correlating the immunoglobulin (Ig) proteomes with the B cell transcriptomes in human fluid and tissue samples, using multiple sclerosis as a paradigm. Oligoclonal Ig bandsExpand
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A novel strategy for quantitative proteomics using isotope‐coded protein labels
Stable isotope labelling in combination with mass spectrometry has emerged as a powerful tool to identify and relatively quantify thousands of proteins within complex protein mixtures. Here weExpand
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Pheromones trigger filamentous growth in Ustilago maydis.
Cell recognition and mating in the smut fungus Ustilago maydis have been proposed to involve specific pheromones and pheromone receptors. The respective structural genes are located in the a matingExpand
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