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Sister-chromatid separation at anaphase onset is promoted by cleavage of the cohesin subunit Scc1
It is shown that Esp1 causes the dissociation of Scc1 from chromosomes by stimulating its cleavage by proteolysis, and a mutant SCC1 is described that is resistant to Esp1-dependent cleavage and which blocks both sister-chromatid separation and the dissociations from chromosomes.
Molecular cloning and expression of brain-derived neurotrophic factor
The full primary structure of brain-derived neurotrophic factor is reported and it is established that these two neurotrophic factors are related both functionally and structurally.
A major transmembrane protein of Golgi-derived COPI-coated vesicles involved in coatomer binding
Biochemical analysis revealed that p23 is enriched in vesicles by a factor of approximately 20, as compared with the donor Golgi fraction, and is present in amounts stoichiometric to the small GTP-binding protein ADP-ribosylation factor (ARF) and coatomer.
Identification of in vivo substrates of the chaperonin GroEL
It is shown that GroEL interacts strongly with a well-defined set of approximately 300 newly translated polypeptides, including essential components of the transcription/translation machinery and metabolic enzymes.
A multiprotein complex mediates the ATP-dependent assembly of spliceosomal U snRNPs
- G. Meister, D. Bühler, R. Pillai, F. Lottspeich, U. Fischer
- BiologyNature Cell Biology
- 1 November 2001
It is shown that assembly of U1 snRNP depends on ATP, the first direct evidence that a complex containing SMN and Gemin2 mediates the active assembly of spliceosomal U snRNPs.
A novel strategy for quantitative proteomics using isotope‐coded protein labels
A novel method, termed isotope‐coded protein label (ICPL), which is capable of high‐throughput quantitative proteome profiling on a global scale, and based on stable isotope tagging at the frequent free amino groups of isolated intact proteins, applicable to any protein sample.
Matching of oligoclonal immunoglobulin transcriptomes and proteomes of cerebrospinal fluid in multiple sclerosis
Compared the Ig transcriptomes of B cells with the corresponding Ig proteomes in CSF samples from four subjects with multiple sclerosis, it is shown that CSF B cells indeed produce the oligoclonal Ig bands.
Tim9, a new component of the TIM22·54 translocase in mitochondria
- A. Adam, M. Endres, C. Sirrenberg, F. Lottspeich, W. Neupert, M. Brunner
- Biology, Computer ScienceThe EMBO journal
- 15 January 1999
Tim9, a new component of the TIM22·54 import machinery, which mediates transport of proteins into the inner membrane of mitochondria, shares sequence similarity with Tim10 and Tim12 and is located in the mitochondrial intermembrane space.
A mechanism for repression of class II gene transcription through specific binding of NC2 to TBP‐promoter complexes via heterodimeric histone fold domains.
It is suggested that binding of the repressor to TRP‐promoter complexes establishes a mechanism that controls the rate of transcription by RNA polymerase II.
Tom5 functionally links mitochondrial preprotein receptors to the general import pore
It is concluded that Tom5 represents a functional link between surface receptors and GIP, and is part of an ‘acid chain’ that guides the stepwise transport of positively charged mitochondrial targeting sequences.