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Classification of difference between inhibition constants of an inhibitor to facilitate identifying the inhibition type
TLDR
By the new decision system, the simulation recovery of uncompetitive inhibitors under CVs of 2% or 5% was improved by four times, but that of competitive or noncompetitive inhibitors was improved slightly; the recovery of L-phenylalanine as an uncompetitive inhibitor of intestinal alkaline phosphatase reached 38%, while the putative decision system lost all.
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Site-Specific PEGylation of Therapeutic Proteins via Optimization of Both Accessible Reactive Amino Acid Residues and PEG Derivatives
TLDR
Optimization of the number and distributions of accessible nonessential reactive residues via molecular engineering can be integrated with the design of large-sized PEG derivatives to achieve site-specific PEGylation of all selected/engineered accessible reactive residues.
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An improved malachite green assay of phosphate: mechanism and application.
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Uricases as Therapeutic Agents to Treat Refractory Gout: Current States and Future Directions
Strategy, Management and Health Policy Enabling Technology, Genomics, Proteomics Preclinical Research Preclinical Development Toxicology, Formulation Drug Delivery, Pharmacokinetics Clinical…
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Assay of serum arylesterase activity by fitting to the reaction curve with an integrated rate equation.
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Approximated maximum adsorption of His-tagged enzyme/mutants on Ni2+-NTA for comparison of specific activities.
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Effects of Modification of Amino Groups with Poly(Ethylene Glycol) on a Recombinant Uricase from Bacillus fastidiosus
After modification with monomethoxyl-poly(ethylene glycol)-5000, a recombinant intracellular uricase from Bacillus fastidiosus ATCC 29604 showed residual activity of about 65%, a thermo-inactivation…
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Characterization of Alcohol Dehydrogenase from Permeabilized Brewer's Yeast Cells Immobilized on the Derived Attapulgite Nanofibers
TLDR
Results show the immobilized ADH retained higher activity over wider ranges of pH and temperature than those of the free, and in bioreduction of EOB mediated by immobilization, the conversion of substrate and enantiomeric excess of product reached 88% and 99.2%, respectively, within 2 h and retained about 42% of the initial activity after eight cycles.
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Kinetic analysis of the lactate-dehydrogenase-coupled reaction process and measurement of alanine transaminase by an integration strategy.
TLDR
A new approach for kinetic analyses of ALT reaction processes and the integration strategy were effective to measure ALT, giving consistent ALT activities in clinical sera.
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Glutathione S‑transferase isozyme alpha 1 is predominantly involved in the cisplatin resistance of common types of solid cancer.
TLDR
The present findings indicated that GSTA1 is a predominant GST isozyme associated with DDP resistance of SGC7901/DDP, A549/D DP and SKOV3/ DDP cells; GSTA 1‑specific inhibitors may be general sensitizers of S GC7901 /DDP and A549 /D DP cells to DDP cytotoxicity through the promotion of cell apoptosis.
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