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Cytoskeletal disruption is the key factor that triggers apoptosis in okadaic acid-treated neuroblastoma cells
TLDR
Results demonstrate that collapse of mitochondrial membrane potential is accessory in the execution of apoptosis, which is directly dependent on cytoskeletal changes. Expand
Azaspiracid-1, a potent, nonapoptotic new phycotoxin with several cell targets.
TLDR
Results indicate that the toxin does not have an apoptotic effect but uses actin for some of its effects, and cytoskeleton seems to be an important cellular target for AZ-1 effect. Expand
Study of cytoskeletal changes induced by okadaic acid in BE(2)-M17 cells by means of a quantitative fluorimetric microplate assay.
TLDR
Results suggest direct interactions of Ser/Thr protein phosphatases with actin-binding proteins in the regulation of actin polymerization, thus indicating that disruption of cytoskeletal structure may be a key mechanism of OA-induced diarrhea. Expand
Fluorescent microplate cell assay to measure uptake and metabolism of glucose in normal human lung fibroblasts.
TLDR
This first report of a fluorimetric microplate assay to assess glucose uptake and metabolism in eukaryotic cells is carried out and it is found that this cell assay is very sensitive, reproducible, provides fast results and graphical display of data. Expand
A fluorescent microplate assay for diarrheic shellfish toxins.
TLDR
This work demonstrates that the fluorescent assay can be used to quantify the amount of okadaic acid in shellfish samples and also is valid for very dilute samples, such as phytoplankton samples. Expand
Development of a F actin-based live-cell fluorimetric microplate assay for diarrhetic shellfish toxins.
A new cytotoxicity assay for detection and quantitation of diarrhetic shellfish toxins (DSP) is presented. This assay is based upon fluorimetric determination of F-actin depolymerization induced byExpand
Characterization of distinct apoptotic changes induced by okadaic acid and yessotoxin in the BE(2)-M17 neuroblastoma cell line.
TLDR
Yessotoxin induced similar changes in BE(2)-M17 cells, although significant differences were found in the time-course and degree of apoptotic events induced by this phycotoxin, indicating a lower potency for yessot toxin when compared with okadaic acid. Expand
Apoptotic events induced by the phosphatase inhibitor okadaic acid in normal human lung fibroblasts.
TLDR
Evidence of apoptotic events induced by okadaic acid in NHLF can be detected by means of sensitive and reliable fluorimetric microplate assays, which indicates that ROS cannot be considered as a hallmark of apoptosis in okadaing acid-treated cells. Expand
A fluorimetric method based on changes in membrane potential for screening paralytic shellfish toxins in mussels.
TLDR
The results indicate that this fluorescent assay is a specific, very sensitive, rapid, and reliable method of monitoring PSP toxin levels in samples from seafood products and toxic algae. Expand
Determination of DSP toxins: comparative study of HPLC and bioassay to reduce the observation time of the mouse bioassay
TLDR
This study considers feasible the reduction to 5 h of the observation times of the mouse bioassay currently established in the actual legislation, as well as the establishment of a DSP-toxin threshold of 2 μg okadaic acid/g hepatopancreas, which regulates the possibility of harvesting and commercialization of contaminated mussels. Expand
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