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Cisgenic plants are similar to traditionally bred plants
![][1] ![][2] ![][3] The testing and release of genetically modified organisms (GMOs)—in particular GM plants—is tightly regulated internationally to prevent any negative effects on theExpand
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Effective production of marker-free transgenic strawberry plants using inducible site-specific recombination and a bifunctional selectable marker gene.
Public concerns about the issue of the environmental safety of genetically modified plants have led to a demand for technologies allowing the production of transgenic plants without selectableExpand
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The potential of virus-induced gene silencing for speeding up functional characterization of plant genes.
Virus-induced gene silencing (VIGS) has been shown to be of great potential in plant reverse genetics. Advantages of VIGS over other approaches, such as T-DNA or transposon tagging, include theExpand
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Functional analysis and expression profiling of HcrVf1 and HcrVf2 for development of scab resistant cisgenic and intragenic apples
Apple scab resistance genes, HcrVf1 and HcrVf2, were isolated including their native promoter, coding and terminator sequences. Two fragment lengths (short and long) of the native gene promoters andExpand
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The effect of exogenously-applied phytohormones on gene transfer efficiency in sugarbeet (Beta vulgaris L.)
Abstract Cellular competence for regeneration and transformation has been monitored in a recalcitrant crop, i.e. sugarbeet ( Beta vulgaris L.). Explants for Agrobacterium inoculation were taken fromExpand
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Using RNA-Seq to assemble a rose transcriptome with more than 13,000 full-length expressed genes and to develop the WagRhSNP 68k Axiom SNP array for rose (Rosa L.)
In order to develop a versatile and large SNP array for rose, we set out to mine ESTs from diverse sets of rose germplasm. For this RNA-Seq libraries containing about 700 million reads were generatedExpand
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Genetic variation, heritability and genotype by environment interaction of morphological traits in a tetraploid rose population
BackgroundGlobal trade has ensured that the ornamental horticulture continues to grow worldwide, with rose hybrids being the most economically important genus (Rosa x hybrida). Due to changes inExpand
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In vitro transformation of plant protoplasts with Ti-plasmid DNA
Genetic engineering requires a procedure for introducing DNA into host cells, followed by integration into the host genome and gene expression. Although several procedures for DNA-medi-ated geneExpand
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A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells
An optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (Beta vulgaris L.). A polyethylene glycol-mediated DNA transformation technique could beExpand
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Tissue-specific expression of the β-glucuronidase reporter gene in transgenic strawberry (Fragaria × ananassa) plants
Abstract. The strawberry (Fragaria spp) is regarded as a false fruit because it originates from the receptacle, which is a non-ovarian tissue. For this reason, fruit-specific promoters isolated fromExpand
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