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Species-Specific and Ubiquitous-DNA-Based Assays for Rapid Identification of Staphylococcus aureus
ABSTRACT Staphylococcus aureus is the cause of serious infections in humans, including endocarditis, deep-seated abscesses, and bacteremia, which lead to toxic and septic shock syndromes. Rapid andExpand
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New Real-Time PCR Assay for Rapid Detection of Methicillin- Resistant Staphylococcus aureus Directly from Specimens Containing a Mixture of Staphylococci
ABSTRACT Molecular methods for the rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) are generally based on the detection of an S. aureus-specific gene target and the mecAExpand
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Correlation between the Resistance Genotype Determined by Multiplex PCR Assays and the Antibiotic Susceptibility Patterns of Staphylococcus aureus andStaphylococcus epidermidis
ABSTRACT Clinical isolates of Staphylococcus aureus (a total of 206) and S. epidermidis (a total of 188) from various countries were tested with multiplex PCR assays to detect clinically relevantExpand
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Development of a PCR Assay for Rapid Detection of Enterococci
ABSTRACT Enterococci are becoming major nosocomial pathogens, and increasing resistance to vancomycin has been well documented. Conventional identification methods, which are based on culturing,Expand
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Development of a PCR Assay for Identification of Staphylococci at Genus and Species Levels
ABSTRACT We have developed a PCR-based assay which allows the detection of staphylococci at the genus level by targeting thetuf gene, which encodes the elongation factor Tu. Degenerate PCR primersExpand
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Species-specific and ubiquitous DNA-based assays for rapid identification of Staphylococcus epidermidis.
Staphylococcus epidermidis is an aerobic gram-positive coccus that is now recognized among the coagulase-negative staphylococci as an etiological agent with an important range of pathogenicity inExpand
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Rapid detection of group B streptococci in pregnant women at delivery.
BACKGROUND Group B streptococcal infections are an important cause of neonatal morbidity and mortality. A rapid method for the detection of this organism in pregnant women at the time of delivery isExpand
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Development of conventional and real-time PCR assays for the rapid detection of group B streptococci.
BACKGROUND Group B streptococci (GBS), or Streptococcus agalactiae, are the leading bacterial cause of meningitis and bacterial sepsis in newborns. Currently available rapid methods to detect GBSExpand
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Rapid Detection of Shiga Toxin-Producing Bacteria in Feces by Multiplex PCR with Molecular Beacons on the Smart Cycler
ABSTRACT We have developed a rapid (1-h) real-time fluorescence-based PCR assay with the Smart Cycler thermal cycler (Cepheid, Sunnyvale, Calif.) for the detection of Shiga toxin-producingExpand
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Rapid Detection of Clostridium difficile in Feces by Real-Time PCR
ABSTRACT Clostridium difficile is the major causative agent of nosocomial antibiotic-associated diarrhea, colitis, and pseudomembranous colitis. The pathogenicity of C. difficile is closely relatedExpand
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