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Endogenous cellulases in animals: isolation of beta-1, 4-endoglucanase genes from two species of plant-parasitic cyst nematodes.
- G. Smant, J. Stokkermans, J. Bakker
- BiologyProceedings of the National Academy of Sciences…
- 28 April 1998
The synthesis of EGases in the esophageal glands of the cyst nematodes Globodera rostochiensis and Heterodera glycines is reported on and it is revealed that the four catalytic domains belong to family 5 of the glycosyl hydrolases.
Use of allele specificity of comigrating AFLP markers to align genetic maps from different potato genotypes
- J. R. van der Voort, P. V. van Zandvoort, J. Bakker
- Biology, MedicineMolecular and General Genetics MGG
- 1 July 1997
The high incidence of corresponding AFLP alleles was confirmed by using an additional set of five primer combinations and the 733 AFLP markers identified provide a valuable reference collection for future mapping studies in potato, which may replace more laborious locus-specific marker techniques.
Gene pool similarities of potato cyst nematode populations assessed by AFLP analysis.
- R. Folkertsma, J. N. Rouppe van der Voort, J. Bakker
- BiologyMolecular plant-microbe interactions : MPMI
The AFLP markers used in this study outnumbered the population characteristics revealed by RAPDs by a factor of five, so to arrive at a reliable pathotype designation of potato cyst nematode populations molecular data and virulence characteristics should be integrated.
The C-terminal KDEL sequence increases the expression level of a single-chain antibody designed to be targeted to both the cytosol and the secretory pathway in transgenic tobacco
The effects of subcellular localization on single-chain antibody (scFv) expression levels in transgenic tobacco was evaluated using an scFv construct of a model antibody possessing different targeting signals and showed that both the secretory signal and the ER retention signal were recognized in the plant cells.
Coordinate expression of antibody subunit genes yields high levels of functional antibodies in roots of transgenic tobacco
The roots of tobacco plants transformed with these constructs produced antibodies that were able to bind antigen in an ELISA, and the construct with divergent promoters showed a better performance than the construct that binds to a fungal cutinase.
Mendelian proof for a gene-for-gene relationship between virulence of Globodera rostochiensis and the H1 resistance gene in Solanum tuberosum ssp. andigena CPC 1673.
Virulence to the HI gene is controlled by a single major recessive gene, and reciprocal crosses showed no evidence of sex-linked inheritance of virulence.
Inter- and intraspecific variation between populations of G. rostochiensis and G. pallida revealed by Random Amplified Polymorphic DNA.
Unweighted pair group method with arithmetic mean analysis of RAPD data showed that G. rostochiensis populations cluster in groups with similar pathotype designations, and no similarity between RAPDData and pathotype classifications of the G. pallida populations was found.
Improving scFv antibody expression levels in the plant cytosol 1
Physiological Diseases Induced by Plant Responses or Products
Phloem transport of carboxyfluorescein through tomato roots infected with Meloidogyne incognita.
Investigating phloem transport through M. incognita infected tomato roots revealed a transport pathway for solutes from the giant cells, via the body of the nematode, out of the root, which may contribute to the higher leakage of solutes known to occur from root-knot nematodes infected roots.