• Publications
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Development of Colorimetric Microtiter Plate Assay for Assessment of Antimicrobials against Acanthamoeba
A 96-well microtiter plate assay, based on the reduction of alamarBlue, is developed and optimized for determination of drug efficacy against two potentially pathogenic species and has been validated by comparison of their relative susceptibilities to chlorhexidine, a drug widely used to treat Acanthamoeba keratitis. Expand
IL‐33 receptor (T1/ST2) signalling is necessary to prevent the development of encephalitis in mice infected with Toxoplasma gondii
This study provides the first evidence of a specific role for IL‐33 receptor signalling in the brain as well as highlighting the requirement of this mechanism in limiting infection with an intracellular parasite. Expand
Sequential expression of macrophage anti‐microbial/inflammatory and wound healing markers following innate, alternative and classical activation
The data explain some apparent contradictions in the literature, demonstrate temporal plasticity in macrophage activation states and define for the first time ‘early’ and ‘late’ markers associated with anti‐microbial/inflammatory and wound healing responses, respectively. Expand
The unusual mitochondrial compartment of Cryptosporidium parvum.
There is no adequate treatment for infection by this parasite and so the elucidation of the role of this organelle and the effective targeting of its functions by antimicrobial agents might provide new treatments for infections by C. parvum. Expand
Salivary nitrite production is elevated in individuals with a higher abundance of oral nitrate‐reducing bacteria
The data from healthy young adults suggest that the abundance of oral nitrate‐reducing bacteria does not influence the generation of NO through the diet, at least when the host has a functional minimum threshold of these microorganisms. Expand
Evidence for mitochondrial-derived alternative oxidase in the apicomplexan parasite Cryptosporidium parvum: a potential anti-microbial agent target.
In spite of the lack of a good candidate for AOX in either the P. falciparum or Toxoplasma gondii genome projects, SHAM and 8-hydroxyquinoline were found to inhibit the growth of these parasites and give insight into the evolution of AOX and demonstrate the potential of targeting the alternative pathway of respiration in apicomplexans. Expand
Toxoplasma gondii dense granule protein 3 (GRA3) is a type I transmembrane protein that possesses a cytoplasmic dilysine (KKXX) endoplasmic reticulum (ER) retrieval motif
It is demonstrated that the previously published sequence for GRA3 is actually an artificial chimera of 2 proteins, one of which possesses a dilysine ‘KKXX’ endoplasmic reticulum (ER) retrieval motif that rationalizes its association with PVM and possibly the host cell ER. Expand
Toll‐like receptor‐4‐mediated macrophage activation is differentially regulated by progesterone via the glucocorticoid and progesterone receptors
  • Leigh A. Jones, J. Anthony, +5 authors Craig W. Roberts
  • Biology, Medicine
  • Immunology
  • 1 September 2008
Compared the ability of progesterone, norgestrel and dexamethasone to modulate macrophage function following stimulation of the Toll‐like receptor‐4 (TLR‐4) ligand lipopolysaccharide (LPS), there is a significant reduction in nitric oxide (NO) production, indicating that this progester one‐mediated effect is through ligation of the glucocorticoid receptor. Expand
Maternal Inheritance and Stage-Specific Variation of the Apicoplast in Toxoplasma gondii during Development in the Intermediate and Definitive Host
It is shown that apicoplasts are present and contain ENR in all T. gondii life cycle stages except microgametes, which will result in maternal inheritance of the organelle. Expand
Resistance of Leishmania donovani to Sodium Stibogluconate Is Related to the Expression of Host and Parasite γ-Glutamylcysteine Synthetase
Results indicate that SSG resistance is associated with manipulation of both host and parasite GSH levels by L. donovani, and indicates that environmental conditions have a profound effect on γ-GCS expression. Expand