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Detection of respiratory syncytial virus using nanoparticle amplified immuno-polymerase chain reaction.
In traditional immuno-polymerase chain reaction (immuno-PCR), a single antibody recognition event is associated with one to three DNA tags, which are subsequently amplified by PCR. Here we describe aExpand
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Bves Modulates Tight Junction Associated Signaling
Blood vessel epicardial substance (Bves) is a transmembrane adhesion protein that regulates tight junction (TJ) formation in a variety of epithelia. The role of TJs within epithelium extends beyondExpand
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  • Open Access
An N‐cadherin–like protein contributes to solute barrier maintenance in cultured endothelium
We investigated the role of cadherins in the solute barrier maintained by endothelial cells in vitro. Cell‐column chromatographic measurement of endothelial barrier showed that reducing normalExpand
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Transfection by Cationic Liposomes Using Simultaneous Single Cell Measurements of Plasmid Delivery and Transgene Expression*
Cationic liposomes are potentially important gene transfer vehicles, although their application has been limited by relatively low efficiency of transgene expression. Single cell quantitativeExpand
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Flow visualization of steady streaming in oscillatory flow through a bifurcating tube
A steady streaming displacement of fluid elements is observed to occur during oscillatory flow through a Y-shaped tube bifurcation model at Womersley and Reynolds numbers that can exist in the humanExpand
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Platelet-derived lysophosphatidic acid decreases endothelial permeability in vitro.
We previously reported that platelets release a soluble factor that decreases the solute permeability of cultured bovine aortic endothelial monolayers. This factor was characterized as heat stable,Expand
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  • Open Access
Platelets and a platelet-released factor enhance endothelial barrier.
The role of platelets in the maintenance of endothelial barrier is examined in an in vitro model of the microvasculature. Human platelets (6,000/microliters) perfused through a cell column ofExpand
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Targeting Expression with Light Using Caged DNA*
In this report, we describe the inactivation and site-specific light induction of plasmid expression using a photosensitive caging compound. Plasmids coding for luciferase were caged withExpand
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  • Open Access
Adenosine decreases permeability of in vitro endothelial monolayers.
The addition of adenosine to perfusates flowing through an in vitro cell-column model of the vasculature decreases the permeability of cell column fetal bovine aortic endothelial monolayers. AtExpand
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Designing for chaos: applications of chaotic advection at the microscale
Chaotic advection can play an important role in efficient microfluidic mixers. We discuss a design paradigm that exploits chaotic advection and illustrate by two recent examples, namely enhancingExpand
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