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Composition and Hierarchical Organisation of a Spider Silk
Albeit silks are fairly well understood on a molecular level, their hierarchical organisation and the full complexity of constituents in the spun fibre remain poorly defined. Here we linkExpand
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Domain Structure of Human Nuclear DNA Helicase II (RNA Helicase A)*
Full-length human nuclear DNA helicase II (NDH II) was cloned and overexpressed in a baculovirus-derived expression system. Recombinant NDH II unwound both DNA and RNA. Limited tryptic digestionExpand
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Characterization of the protein components of Nephila clavipes dragline silk.
Spider silk is predominantly composed of structural proteins called spider fibroins or spidroins. The major ampullate silk that forms the dragline and the cobweb's frame threads of Nephila clavipesExpand
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Effect of 4-quinolones and novobiocin on calf thymus DNA polymerase alpha primase complex, topoisomerases I and II, and growth of mammalian lymphoblasts.
The influence of ciprofloxacin, nalidixic acid, norfloxacin, novobiocin, and ofloxacin on elements of eucaryotic DNA replication was investigated in vitro. Each of the 4-quinolones, when present inExpand
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Immunoaffinity-purified DNA polymerase alpha displays novel properties.
The purification and characterization of a novel and more intact form of the DNA polymerase alpha-primase complex from calf thymus are described. The polymerase-primase was enriched 10,000-fold toExpand
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Protein-Protein Interactions of the Primase Subunits p58 and p48 with Simian Virus 40 T Antigen Are Required for Efficient Primer Synthesis in a Cell-free System*
DNA polymerase α-primase (pol-prim, consisting of p180-p68-p58-p48), and primase p58-p48 (prim2) synthesize short RNA primers on single-stranded DNA. In the SV40 DNA replication system, only pol-primExpand
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Differential polymerization of the two main protein components of dragline silk during fibre spinning
Spider silks are some of the strongest materials found in nature1,2. Achieving the high tensile strength and elasticity of the dragline of orb-weaving spiders, such as Nephila clavipes3,4,5, is aExpand
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The conserved C-termini contribute to the properties of spider silk fibroins.
Spider silk fibroins can adopt different structural states at high protein concentrations. They are soluble within the spinning dope of the glands, but readily converted into insoluble polymers uponExpand
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Molecular Cloning of the Gene Encoding Nuclear DNA Helicase II. A BOVINE HOMOLOGUE OF HUMAN RNA HELICASE A AND DROSOPHILA Mle PROTEIN (*)
Nuclear DNA helicase II (NDH II) unwinds both DNA and RNA (Zhang, S., and Grosse, F.(1994) Biochemistry 33, 3906-3912). Here, we report on the molecular cloning and sequence determination of theExpand
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Lactate dehydrogenase and glyceraldehyde-phosphate dehydrogenase are single-stranded DNA-binding proteins that affect the DNA-polymerase-alpha-primase complex.
Affinity chromatography on double-stranded (ds) and single-stranded (ss) DNA-cellulose columns was employed to find analogs of the Escherichia coli and T4 single-stranded DNA binding proteins (SSBExpand
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