• Publications
  • Influence
Sustainability of transgenic insecticidal cultivars: integrating pest genetics and ecology.
  • F. Gould
  • Biology, Medicine
    Annual review of entomology
  • 1998
Theoretical and practical issues involved in implementing strategies to delay pest adaptation to insecticidal cultivars are reviewed and emphasis is placed on examining the "high dose"/refuge strategy that has become the goal of industry and regulatory authorities.
Identification of a Gene Associated with Bt Resistance in Heliothis virescens
This work shows that disruption of a cadherin-superfamily gene by retrotransposon-mediated insertion was linked to high levels of resistance to the Bt toxin Cry1Ac in the cotton pest Heliothis virescens, enabling efficient DNA-based screening for resistant heterozygotes by directly detecting the recessive allele.
Selection and Genetic Analysis of a Heliothis virescens (Lepidoptera: Noctuidae) Strain with High Levels of Resistance to Bacillus thuringiensis Toxins
Results of this selection experiment indicate that the initial frequency of this resistance trait could be approximately 10 -3 , but field tests will be needed to confirm this rough estimate.
Initial frequency of alleles for resistance to Bacillus thuringiensis toxins in field populations of Heliothis virescens.
A single-pair mating technique greatly increases the efficiency of detecting recessive resistance alleles and could be useful in estimating resistance allele frequencies in other insects exposed to transgenic insecticidal crops or conventional insecticides.
Gene drive systems for insect disease vectors
The potential of natural gene drive systems for spreading genes that can block the transmission of insect-borne pathogens are explored and the artificial constructs that could be envisaged for this purpose are discussed.
Determining the public health impact of physiological and behavioral insecticide resistance is an urgent priority if the authors are to maintain the significant gains made in reducing malaria morbidity and mortality.
Manipulating natural enemies by plant variety selection and modification: a realistic strategy?
A better understanding of plant/pest/natural enemy evolution is necessary to predict how to combine natural enemies and plant resistance for the best long-term results.
Functional characterization of pheromone receptors in the tobacco budworm Heliothis virescens
Functional analyses of candidate Heliothis virescens pheromone odorant receptors (HvORs) were conducted using heterologous expression in Xenopus oocytes. HvOR6 was found to be highly tuned to
Spread of resistance in spatially extended regions of transgenic cotton : Implications for management of Heliothis virescens (Lepidoptera: Noctuidae)
It is found that the spatial scale and the temporal pattern of refuges can have a strong effect on the development of resistance to B. thuringiensis in transgenic cotton and delays in larval development time on Bt cotton may either increase or decrease the rate of resistance development, depending on complex interactions with the length of the growing season.
Overexpression of the Bacillus thuringiensis (Bt) Cry2Aa2 protein in chloroplasts confers resistance to plants against susceptible and Bt-resistant insects.
The results suggest that plants expressing high levels of a nonhomologous Bt protein should be able to overcome or at the very least, significantly delay, broad spectrum Bt-resistance development in the field.