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Cloning and expression of the rat BACE1 promoter
The pathogenic processing of the amyloid precursor protein (APP) into β‐amyloid peptides, which give rise to β‐amyloid plaques in the brains of Alzheimer's disease patients, requires the enzymaticExpand
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Hepatocellular expression of glutamine synthetase: an indicator of morphogen actions as master regulators of zonation in adult liver.
Glutamine synthetase (GS) has long been known to be expressed exclusively in pericentral hepatocytes most proximal to the central veins of liver lobuli. This enzyme as well as its peculiarExpand
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Treatment of cirrhotic rats with L-ornithine-L-aspartate enhances urea synthesis and lowers serum ammonia levels.
CCl4-induced cirrhosis of rats was used for studying the influence of L-ornithine-L-aspartate (OA) on hyperammonemia. OA given to cirrhotic rats (2 g/kg daily) for 2 wk slightly increased net bodyExpand
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Simple and fast microscale procedure for transfection and quantification of reporter gene expression in eukaryotic cells.
The quantification of the expression levels of commonly used reporter genes (e.g. β-galactosidase or luciferase) is an important and commonly used procedure in many laboratories working withExpand
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Heterogeneous (positional) expression of hepatic glutamine synthetase: features, regulation and implications for hepatocarcinogenesis.
Glutamine synthetase expression in liver parenchyma is restricted to a small population of pericentral hepatocytes surrounding the central veins. Studies on the development of this heterogeneousExpand
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Cis-Regulatory Sequences from the First Intron of the Rat Glutamine Synthetase Gene Are Involved in Hepatocyte Specific Expression of the Enzyme
In order to identify regulatory elements involved in the hepatocyte specific expression of the enzyme glutamine synthetase [GS (E.C.] we analyzed the first intron of the rat GS gene. AExpand
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Cell-cell interactions in the regulation of the expression of hepatic enzymes
The expression of enzymes and other proteins in liver parenchyma is heterogeneous and shows distinct features depending on whether all or only a subset of hepatocytes are involved. Recent advances inExpand
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Transient transfection of primary cultured hepatocytes using CaPO4/DNA precipitation.
We present a detailed protocol for the transient transfection of non-proliferating primary cultured hepatocytes that is easily reproducible. Using a modification of the classical CaPO4/DNAExpand
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Gene transfer and expression.
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Post-transcriptional inhibition of glutamine synthetase induction in rat liver epithelial cells exerted by conditioned medium from rat hepatocytes.
Recently, a soluble factor produced by primary periportal hepatocytes and different from glutamine was found to completely block induction of glutamine synthetase (GS) by dexamethasone (DEX) in theExpand
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