F. Gast

Publications31
h-index15
Citations627
Highly Influential Citations26
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A single nucleotide substitution converts potato spindle tuber viroid (PSTVd) from a noninfectious to an infectious RNA for nicotiana tabacum.
TLDR
The sequence analysis of PSTVd NT revealed a single nucleotide substitution from C-->U at position 259, which might be involved in protein binding and in viroid processing in both tobacco and tomato.
Secondary structure probing of potato spindle tuber viroid (PSTVd) and sequence comparison with other small pathogenic RNA replicons provides evidence for central non-canonical base-pairs, large…
TLDR
Non-denaturing gel electrophoresis shows that linear (non-circular) unit-length (+)strand RNA molecules of a lethal PSTVd variant were produced which are able to initiate typical disease symptoms when inoculated into tomato.
The GTP-binding domain of McrB: more than just a variation on a common theme?
TLDR
A mutational analysis of the putative GTP-binding domain of McrB is reported, finding that it is not a typical G protein, but defines a new subfamily within the superfamily of G TP-binding proteins, together with similar prokaryotic proteins of as yet unidentified function.
The Recognition of Methylated DNA by the GTP-Dependent Restriction Endonuclease McrBC Resides in the N-Terminal Domain of Mcr B
TLDR
Gel retardation assays with wild-type and mutant McrB reveal that single 5'-Pu(m)C sites in DNA can be sufficient to elicite binding by McRB, suggesting that the two activities are coupled in the full-length protein.
The DNA/RNA non-specific Serratia nuclease prefers double-stranded A-form nucleic acids as substrates.
TLDR
Cleavage experiments with various polynucleotides, including a viroid RNA and a specifically designed 167 bp DNA, confirm that double-stranded A-form nucleic acids are preferentially attacked by Serratia nuclease.
Determination of the angle between the anticodon and aminoacyl acceptor stems of yeast phenylalanyl tRNA in solution.
TLDR
This work has constructed a yeast tRNA(Phe) heteroduplex RNA molecule in which the anticodon and acceptor stems of the tRNA have each been extended by approximately 70 base pairs and yields an interstem angle essentially identical to the corresponding angle observed in the crystal under similar buffer and temperature conditions.
The microscopy cell (MicCell), a versatile modular flowthrough system for cell biology, biomaterial research, and nanotechnology
TLDR
A range of applications are presented, including single-molecule analysis by fluorescence correlation spectroscopy (FCS), manipulation of single molecules for nanostructuring by hydrodynamic flow fields or the action of motor proteins, generation of concentration gradients, trapping and stretching of live cells using optical fibers precisely mounted in the PDMS layer.
Immobilization of sugar-non-specific nucleases by utilizing the streptavidin--biotin interaction.
The elongation factor Tu from Escherichia coli, aminoacyl-tRNA, and guanosine tetraphosphate form a ternary complex which is bound by programmed ribosomes.
The dimerization domain of potato spindle tuber viroid, a possible hallmark for infectious RNA.
TLDR
The dimerization hairpin of viroid RNA represents a unique conformational signal that is homologous to similar regions in the human immunodeficiency virus and other retroviruses.
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