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Substrate-gel electrophoresis for composition and molecular weight of proteinases or proteinaceous proteinase inhibitors.
A rapid, sensitive, and generally applicable substrate-sodium dodecyl sulfate-polyacrylamide gel electrophoresis method for detection of proteinases or proteinaceous protease inhibitors in biologicalExpand
Isolation and characterization of trypsin from pyloric caeca of Monterey sardine Sardinops sagax caerulea.
Trypsin from pyloric caeca of Monterey sardine was purified by fractionation with ammonium sulfate, gel filtration, affinity and ionic exchange chromatography and general characteristics resemble those of trypsins from other fish. Expand
Characterization of acidic proteolytic enzymes from Monterey sardine (Sardinops sagax caerulea) viscera
Abstract Total enzyme activity of whole viscera, and partial characterization of acidic proteases from Monterey sardine viscera are presented. Major proteolytic activity in alkali (pH 10) and minorExpand
The digestive proteases of langostilla (pleuroncodes planipes, decapoda): their partial characterization, and the effect of feed on their composition
The composition of proteins and molecules bearing protease activity from the hepatopancreas and stomach of both fed and starved animals was similar, indicating proteases are not induced but constitutive. Expand
Invertebrate trypsins: a review
Recent advances in the knowledge of invertebrate trypsin synthesis, regulation, genetics, catalytic characteristics; structure, evolution, as well as inhibition, especially in non-Drosophilidae insects and in some crustaceans are reviewed. Expand
Enzymes with Peptidase and Proteinase Activity from the Digestive Systems of a Freshwater and a Marine Decapod
Results show the decapod chymotrypsins possess different catalytic properties which do not include inhibition by tosyl-Phe chloromethyl ketone, and decapidase and proteinase activities from the digestive systems of langostilla and crayfish were evaluated. Expand
Digestive proteinases of red shrimp Pleoticus muelleri (Decapoda, Penaeoidea): partial characterization and relationship with molting.
Comparison of the protease-specific activity of the hepatopancreas of some species indicated a relationship between digestive enzyme activity and feeding habits of the shrimp. Expand
Penaeus vannamei isotrypsins: purification and characterization.
Three isotrypsins from digestive gland of Penaeus vannamei were purified and characterized by molecular, biochemical and kinetic parameters, finding Isoform C has higher physiological efficiency and specific activity, lower K(m), and requires higher concentrations of Ca(+2) to reach the same activity as the other two isotryPSins. Expand
Stability and effects of organic solvents on endopeptidases from the gastric fluid of the marine crab Cancer pagurus
Activities of proteolytic enzymes, represented by azocasein digestion (total protease), trypsin, and chymotrypsin from the gastric fluid of the marine crab, Cancer pagurus, were evaluated forExpand
pH-stat method to predict protein digestibility in white shrimp (Penaeus vannamei)
A pH-stat assay using an enzyme fraction from shrimp hepatopancreas was developed to evaluate the degree of hydrolysis (DH) and estimate protein quality in menhaden, anchovy, white fish, tuna waste, soybean protein, and langostilla meals and predicted the protein quality better than chemical analysis. Expand