Glutamate: A Neurotransmitter in Mammalian Brain
- F. Fonnum
- Biology, ChemistryJournal of Neurochemistry
- 1 January 1984
The evidence for glutamate as a transmitter at the locust neuromuscular junction has recently been carefully evaluated by Usherwood (1981), and it is shown that mechanisms exist that will terminate transmitter action rapidly.
A rapid radiochemical method for the determination of choline acetyltransferase
- F. Fonnum
- Chemistry, BiologyJournal of Neurochemistry
- 1 February 1975
It is shown that the method for isolating labelled ACh by liquid cation exchange using sodium tetraphenylboron (Kalignost) in ethyl butyl ketone can be simplified considerably by using the scintillation mixture as extraction solvent and carrying out the extraction directly in theScintillation vial.
Evaluation of the probes 2',7'-dichlorofluorescin diacetate, luminol, and lucigenin as indicators of reactive species formation.
An In Vivo Model for Studying Function of Brain Tissue Temporarily Devoid of Glial Cell Metabolism: The Use of Fluorocitrate
- R. Paulsen, A. Contestabile, L. Villani, F. Fonnum
- Biology, MedicineJournal of Neurochemistry
- 1 May 1987
The use of microinjection of 1 nmol of fluorocitrate into the neostria‐tum of the rat to provide a model for studying transmitter amino acid metabolism in brain devoid of glial cell activity is discussed.
Use of fluorocitrate and fluoroacetate in the study of brain metabolism
Fluoroacetate and its toxic metabolite fluorocitrate cause inhibition of aconitase and it is the latter that has received most attention in the study of glial‐neuronal interactions, since glutamine is an important precursor for transmitter glutamate and GABA.
Selective inhibition of glial cell metabolism in vivo by fluorocitrate
Radiochemical micro assays for the determination of choline acetyltransferase and acetylcholinesterase activities.
- F. Fonnum
- Biology, ChemistryBiochemical Journal
- 1 November 1969
The methods were tested with samples from central and peripheral nervous tissues and purified enzymes and the values of choline acetyltransferase activity obtained in the presence of sodium cyanide or EDTA and synthetic acetyl-CoA were similar to those obtained with acetyl -CoA synthesized in situ.
The effect of brominated flame retardants on neurotransmitter uptake into rat brain synaptosomes and vesicles
Uptake of l‐Glutamate into Rat Brain Synaptic Vesicles: Effect of Inhibitors that Bind Specifically to the Glutamate Transporter
The two most efficient inhibitors of the vesicular uptake of glutamate were the dyes Evans blue and Chicago Skye Blue 6B, which are structurally related to glutamate and were competitive inhibitors in the nanomolar range.
Neurotoxicity of the pentabrominated diphenyl ether mixture, DE-71, and hexabromocyclododecane (HBCD) in rat cerebellar granule cells in vitro
The results indicate that the lower brominated PBDEs have a higher potency of bioaccumulation than HBCD, and that both compounds have a neurotoxic potential in vitro.