F. Durst

Publications116
h-index40
Citations5,290
Highly Influential Citations233
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Cytochrome P450
TLDR
It is shown that in allylic hydroxyl ations (catalyzed by either model metalloporphyrins or cytochrome P450) rehydridization could occur to yield multiple products.
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Functional analysis of the LACERATA gene of Arabidopsis provides evidence for different roles of fatty acid ω-hydroxylation in development
TLDR
It is indicated that LCR-dependent ω-hydroxylation of fatty acids could be implicated in the biosynthesis of cutin in the epidermis and in preventing postgenital organ fusions.
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CYP86A1 from Arabidopsis thaliana encodes a cytochrome P450-dependent fatty acid omega-hydroxylase.
TLDR
Genomic organization analyzed by Southern blot suggested a single gene encoding CYP86A1 in A. thaliana, which was found to catalyze the omega-hydroxylation of saturated and unsaturated fatty acids with chain lengths from C12 to C18 but not of hexadecane.
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Isolation and sequence of a cDNA encoding the Jerusalem artichoke cinnamate 4-hydroxylase, a major plant cytochrome P450 involved in the general phenylpropanoid pathway.
TLDR
According to current criteria, the purification of this enzyme from Mn(2+)-induced Jerusalem artichoke (Helianthus tuberosus L.) tuber tissues qualifies as a member of a new P450 family, and this P450 protein exhibits the most similarity (28% amino acid identity) with avocado CYP71, but also good similarity with CYP17 and CYP21, or with CyP1 and CyP2 families.
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Diversity and Evolution of Plant P450 and P450-Reductases
TLDR
There appears to be as much divergence between plant families as there is between plant and animal, fungal or microbial families.
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Arabidopsis cyp51 Mutant Shows Postembryonic Seedling Lethality Associated with Lack of Membrane Integrity1[w]
TLDR
The results demonstrate that the Arabidopsis CYP51A2 gene encodes a functional obtusifoliol 14α-demethylase enzyme and plays an essential role in controlling plant growth and development by a sterol-specific pathway.
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Characterization of recombinant plant cinnamate 4-hydroxylase produced in yeast. Kinetic and spectral properties of the major plant P450 of the phenylpropanoid pathway.
TLDR
Results indicate that the microsomal system constituted by the overexpressed yeast P450 reductase and CA4H is characterized by a 1:1 coupling between NADPH oxidation and cinnamate hydroxylation and by one of the highest turnover numbers reported for an NADPH-dependent P450 reaction.
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Catalytic properties of the plant cytochrome P450 CYP73 expressed in yeast. Substrate specificity of a cinnamate hydroxylase.
TLDR
Comparison of the kinetic parameters of CYP73 for its various substrates showed that cinnamate was by far the best substrate of this P450, and the physiological and toxicological significance of these observations are discussed.
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The Major Protein of Guayule Rubber Particles Is a Cytochrome P450
TLDR
Together, these factors place RPP in the CYP74 family of P450s and establish it as the first P450 localized in rubber particles and the first eukaryotic P450 to be identified outside endoplasmic reticulum, mitochondria, or plastids.
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CYP94A5, a new cytochrome P450 from Nicotiana tabacum is able to catalyze the oxidation of fatty acids to the omega-alcohol and to the corresponding diacid.
TLDR
A cytochrome P450 able to catalyze the complete set of reactions oxidizing a terminal methyl group to the corresponding carboxyl is described, which is enantioselective: after incubation of a synthetic racemic mixture of 9,10-epoxystearic acid, the chirality of the residual epoxide was 40/60 in favor of 9R,10S enantiomer.
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