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A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis
TLDR
A large ribonucleoprotein (RNP) complex from Saccharomyces cerevisiae that contains the U3 snoRNA and 28 proteins is purified and it is suggested that this complex may correspond to the terminal knobs present at the 5′ ends of nascent pre-rRNAs.
Human H/ACA Small Nucleolar RNPs and Telomerase Share Evolutionarily Conserved Proteins NHP2 and NOP10
TLDR
Immunofluorescence microscopy experiments showed that hGAR1, hNOP10, and hNHP2 are localized in the dense fibrillar component of the nucleolus and in Cajal (coiled) bodies.
In Vitro Assembly of Human H/ACA Small Nucleolar RNPs Reveals Unique Features of U17 and Telomerase RNAs
TLDR
Antibodies that specifically recognize the human GAR1 (hGAR1) protein could immunoprecipitate H/ACA snoRNAs and hTR from HeLa cell extracts, which demonstrates that hGAR 1 is a component of H/ ACA sno RNPs and telomerase in vivo.
Conserved composition of mammalian box H/ACA and box C/D small nucleolar ribonucleoprotein particles and their interaction with the common factor Nopp140.
TLDR
It is proposed that Nopp140 functions as a chaperone of snoRNPs in yeast and vertebrate cells, raising the possibility of a feedback mechanism between rRNA modification and transcription.
Effects of dyskeratosis congenita mutations in dyskerin, NHP2 and NOP10 on assembly of H/ACA pre-RNPs.
TLDR
The results suggest that, in addition to major defects in the telomere/telomerase pathways, some of the disorders occurring in DC may be caused by alteration of most H/ACA RNPs, or by only a subset of them.
A new system for naming ribosomal proteins.
Nucleolar localization signals of Box H/ACA small nucleolar RNAs
TLDR
Analysis of U17, U64 and U65 has revealed that disruption of either of the conserved sequence elements, Box H or Box ACA, eliminates nucleolar localization, and it is demonstrated that telomerase RNA is also targeted to the nucleolus by a Box ACA‐dependent mechanism.
A nucleolar protein related to ribosomal protein L7 is required for an early step in large ribosomal subunit biogenesis.
TLDR
It is proposed that Rlp7p may act as a specificity factor that binds precursor rRNAs and tethers the enzymes that carry out the early 5' to 3' exonucleolytic reactions that generate the mature r RNAs.
Dyskeratosis congenita mutations in the H/ACA domain of human telomerase RNA affect its assembly into a pre-RNP.
TLDR
It is shown that human NAF1 cannot bind directly to the H/ACA domain of hTR, and requires the core trimer dyskerin-NOP10-NHP2 to be efficiently incorporated into the pre-RNP, the first evidence that DC-related mutations of h TR C408G and Delta378-451 severely impair pre- RNP assembly.
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