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Viroids and viroid-host interactions.
TLDR
Although they induce symptoms in plants similar to those accompanying virus infections, viroids have unique structural, functional, and evolutionary characteristics and could exert their pathogenic effects via RNA silencing. Expand
Small RNAs containing the pathogenic determinant of a chloroplast-replicating viroid guide the degradation of a host mRNA as predicted by RNA silencing.
TLDR
It is determined that two PLMVd-sRNAs containing the PC-associated insertion target for cleavage the mRNA encoding the chloroplastic heat-shock protein 90 (cHSP90), thus implicating RNA silencing in the modulation of host gene expression by a viroid. Expand
Viroids: how to infect a host and cause disease without encoding proteins.
TLDR
Support is provided for the view that symptoms could be produced by viroid-derived small RNAs -generated by the host defensive machinery- targeting specific host mRNA or DNA sequences for post-transcriptional or transcriptional gene silencing, respectively, a hypothesis that could also explain pathogenesis of non-protein-coding satellite RNAs. Expand
Deep Sequencing of Viroid-Derived Small RNAs from Grapevine Provides New Insights on the Role of RNA Silencing in Plant-Viroid Interaction
TLDR
These findings show that different Dicer-like enzymes (DCLs) target viroid RNAs, preferentially accessing to the same viroid domains, indicating more complex scenarios than previously thought for both vd-sRNAs genesis and possible interference with host gene expression. Expand
Local expression of enzymatically active class I beta-1, 3-glucanase enhances symptoms of TMV infection in tobacco.
TLDR
The results provide the first direct evidence that a biological function of a plant beta-1,3-glucanase depends on its catalytic activity, and supports the view that GLU I induced locally during infection helps to promote cell-to-cell movement of virus by hydrolyzing callose. Expand
Identification and characterization of a novel geminivirus with a monopartite genome infecting apple trees.
TLDR
The cloned viral genome was infectious in several herbaceous plants, thus confirming it was complete and biologically active, although no symptoms were observed in these experimental hosts, and phylogenetic analyses did not support the inclusion of this novel species in any of the established genera in the family Geminiviridae. Expand
Taxonomy of the order Bunyavirales: update 2019
TLDR
The updated taxonomy of the order Bunyavirales now accepted by the International Committee on Taxonomy of Viruses (ICTV) is presented. Expand
Specific Argonautes Selectively Bind Small RNAs Derived from Potato Spindle Tuber Viroid and Attenuate Viroid Accumulation In Vivo
TLDR
In leaves of Nicotiana benthamiana infected by potato spindle tuber viroid, the endogenous AGO1 and distinct AGOs from Arabidopsis thaliana that were overexpressed were associated with vd-sRNAs displaying the same properties previously established for endogenous and viral small RNAs, supporting their role in antiviroid defense. Expand
Identification and characterization of privet leaf blotch-associated virus, a novel idaeovirus.
TLDR
Transient expression of a recombinant PrLBaV p38 fused to green fluorescent protein in leaves of Nicotiana benthamiana, coupled with confocal laser scanning microscopy assays showed that it localizes at cell plasmodesmata, strongly supporting its involvement in viral movement/trafficking and providing the first functional characterization of an idaeovirus encoded protein. Expand
Peach latent mosaic viroid variants inducing peach calico (extreme chlorosis) contain a characteristic insertion that is responsible for this symptomatology.
TLDR
Data demonstrate that the agent of PC is PLMVd and direct support that the 12- to 13-nt insertion contains the PC pathogenicity determinant was obtained by its removal through site-directed mutagenesis from one of the PC-inducing variants. Expand
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