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New vectors for combinatorial deletions in yeast chromosomes and for gap‐repair cloning using ‘split‐marker’ recombination
TLDR
A novel transformation strategy, based on ‘split‐marker’ recombination, is developed, which allows generation of chromosomal deletions and direct gene cloning in yeast and is applied to the analysis of a few open reading frames of chromosome XI.
Intracellular adaptation of Brucella abortus.
TLDR
Insight is provided into mechanisms utilized by Brucella to establish intracellular infections by comparing proteomes of virulent and attenuated strains of the species.
Antibacterial Activity of the Lactoperoxidase System on Listeria monocytogenes in Trypticase Soy Broth, UHT Milk and French Soft Cheese.
TLDR
The results of this study suggest that the lactoperoxidase system may be effective in dairy products as a safety factor to assist in the inhibition of L. monocytogenes.
Cloning and characterization of cDNA probes for the analysis of metallothionein gene expression in the Mediterranean bivalves: Ruditapes decussatus and Cerastoderma glaucum
TLDR
The induction of metallothionein gene expression in CdCl2 treated bivalves was confirmed by dot blot analysis and suggests a potential specific tissue expression rate.
Transcriptional response of stress-regulated genes to cadmium exposure in the cockle Cerastoderma glaucum from the gulf of Gabès area (Tunisia)
TLDR
Investigation of cadmium effects on key messenger RNA expression by qPCR in the cockle Cerastoderma glaucum finds MT, HSP70, CAT, and CuZnSOD seem to be relevant biomarkers of Cd exposure because their mRNA levels increase upon exposure and they are highly correlated to Cd accumulation in tissues.
Transcriptional response of stress-regulated genes to industrial effluent exposure in the cockle Cerastoderma glaucum
TLDR
Gene transcription monitoring performed on seven genes potentially involved in the tolerance to metal exposure showed that for both exposures, mechanisms are rapidly and synchronically settled down to prevent damage to cellular components.
Screening of Helicoverpa armigera Mobilome Revealed Transposable Element Insertions in Insecticide Resistance Genes
TLDR
The cotton bollworm Helicoverpa armigera Hübner is an important pest of many crops that has developed resistance to almost all groups of insecticides used for its management, and Insecticide resistance was often related to Transposable Element (TE) insertions near specific genes.
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