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Hypoxia inducible factors regulate pluripotency and proliferation in human embryonic stem cells cultured at reduced oxygen tensions
It is demonstrated that a low oxygen tension is preferential for the maintenance of a highly proliferative, pluripotent population of hES cells. Expand
Non-invasive amino acid turnover predicts human embryo developmental capacity.
Non-invasive amino acid profiling has the potential to select developmentally competent single embryos for transfer, thereby increasing the success rate and eliminating multiple births in IVF. Expand
Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover.
Non-invasive assay of amino acid turnover has the potential to improve significantly the prospective selection of the most viable embryos, or single embryo, for replacement in an IVF cycle. Expand
Oxygen uptake and carbohydrate metabolism by in vitro derived bovine embryos.
The data revealed that bovine embryos were dependent on oxidative phosphorylation for energy (ATP) production at all stages of pre-elongation development, with perhaps a shift in dependence towards glycolysis in conjunction with compaction. Expand
Oxygen consumption and energy metabolism of the early mouse embryo
The data are consistent with a switch from a metabolism dependent on aerobic respiration during early preimplantation stages to one dependent on both oxidative phosphorylation and aerobic glycolysis at the blastocyst stage, a pattern which is maintained on days 6.5 and 7.5. Expand
Energy metabolism of the inner cell mass and trophectoderm of the mouse blastocyst.
  • F. D. Houghton
  • Biology, Medicine
  • Differentiation; research in biological diversity
  • 1 February 2006
The pluripotent cells of the ICM display a relatively quiescent metabolism in comparison with that of the TE, which is likely to be the Na(+), K(+)ATPase (sodium pump enzyme) located on the TE basolateral membrane. Expand
Effect of Inhibiting Nitric Oxide Production on Mouse Preimplantation Embryo Development and Metabolism1
The data suggest a physiological role for NO in mouse preimplantation metabolism and development and one possibility is that NO may limit oxygen consumption at the blastocyst stage at the level of mitochondrial cytochrome c oxidase. Expand
Expression of genes involved in early cell fate decisions in human embryos and their regulation by growth factors.
Examination of expression in human preimplantation embryos of a number of genes known to be critical for early development of the murine embryo improves knowledge of the similarities between human and murine embryos and the influence of GFs on human embryo gene expression. Expand
HIF-2α Regulates NANOG Expression in Human Embryonic Stem Cells following Hypoxia and Reoxygenation through the Interaction with an Oct-Sox Cis Regulatory Element
A novel role of HIF-2α is uncovered as a direct regulator of key transcription factors controlling self-renewal in hESCs but also in the induction of epigenetic modifications ensuring a euchromatic conformation which enhances the regenerative potential of these cells. Expand
Association between amino acid turnover and chromosome aneuploidy during human preimplantation embryo development in vitro.
The association between the metabolism, cytogenetic composition and health of human embryos in vitro is demonstrated and gender significantly affected the metabolism of tryptophan, leucine and asparagine by cleavage-stage embryos. Expand