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Ionic liquids dispersive liquid-liquid microextraction and high-performance liquid chromatographic determination of irbesartan and valsartan in human urine.
The developed IL-DLLME-HPLC method coupled with high-performance liquid chromatography was successfully applied for evaluation of the urine irbesartan and valsartan profile following oral capsules administration. Expand
Structural basis of transcobalamin recognition by human CD320 receptor
The crystal structure of human holo-TC in complex with the extracellular domain of CD320 is presented, visualizing the structural basis of the TC-CD320 interaction and significantly reduced in vitro affinity of TC for CD320 at low pH is demonstrated. Expand
Understanding chemical reactivity for homo- and heterobifunctional protein cross-linking agents.
The protein structure itself, especially the distances between target amino acid residues, was found to be a determining factor for the cross-linking efficiency, and the reactive groups of the chemical cross-linker also play an important role; a higher cross- linking reaction efficiency was found for maleimides compared to 2-pyrimidyldithiols. Expand
Anaerobic degradation of chlorothalonil in four paddy soils.
The 4-hydroxy-2,5,6- trichloroisophthalonitrile (HTI), one of the major metabolites of CTN degradation, has shown the significant inhibitation to the anaerobic CTN biodegradation when its residual level is over 0.1 μg g(-1). Expand
Multiple Substrate Usage of Coxiella burnetii to Feed a Bipartite Metabolic Network
The data reflect multiple and differential substrate usages by C. burnetii in a bipartite-type metabolic network, resembling the overall topology of the related pathogen Legionella pneumophila and could benefit the metabolic capacities of the pathogens also as a trait to adapt for replication under intracellular conditions. Expand
Determination of Chlorpyrifos in Human Blood by Gas Chromatography-Mass Spectrometry
Gas chromatography-mass spectrometry method was developed for the qualitative and quantitative analyses of chlorpyrifos in human blood samples. The chlorpyrifos and parathion (internal standard) inExpand
Applying mass spectrometry to study non-covalent biomolecule complexes.
Both approaches are capable of studying non-covalently bound biomolecules even in quite challenging systems, such as membrane protein complexes. Expand
Analysis of phenolic pollutants in human samples by high performance capillary electrophoresis based on pretreatment of ultrasound-assisted emulsification microextraction and solidification of
The developed UAEM-SFO-HPCE method has a great potential in routine residual analysis of trace PCs in biological samples and is based on microextraction of target analytes in less-toxic organic solvent under assistance of ultrasound. Expand
High-mass matrix-assisted laser desorption ionization-mass spectrometry of integral membrane proteins and their complexes.
A standard matrix-assisted laser desorption/ionization (MALDI) protocol, if used in combination with a high-mass detector, allows straightforward mass spectrometric measurements of integral membrane proteins and their complexes, directly following purification in detergent solution. Expand
Ultrathin-shell boron nitride hollow spheres as sorbent for dispersive solid-phase extraction of polychlorinated biphenyls from environmental water samples.
Results demonstrated that the hexagonal boron nitride-based material has significant potential as a sorbent for organic pollutant extraction from environmental water samples. Expand