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A novel homeodomain-encoding gene is associated with a large CpG island interrupted by the myotonic dystrophy unstable (CTG)n repeat.
RT-PCR analysis shows that this gene, which is called DM locus-associated homeodomain protein (DMAHP), is expressed in a number of human tissues, including skeletal muscle, heart and brain. Expand
Cloning and sequencing of cDNAs encoding the human hepatocyte nuclear factor 4 indicate the presence of two isoforms in human liver.
Hepatocyte nuclear factor 4 (HNF-4) is a key transcription factor involved in the specific expression of many genes in liver and intestine. Sequences of cDNAs coding for HNF-4 have been establishedExpand
Characterization of the chromosomal protein MC1 from the thermophilic archaebacterium Methanosarcina sp. CHTI 55 and its effect on the thermal stability of DNA.
The studies indicate that one molecule of protein MC1 protects eight base pairs of DNA, which is close to the normal level for a deoxyribonucleoprotein complex. Expand
Construction of a mouse yeast artificial chromosome library in a recombination–deficient strain of yeast
A new generation yeast artificial chromosome library from female C57BL/10 mice in a recombination–deficient strain of Saccharomyces cerevisiae carrying a mutation in the RAD52 gene should enable a long–term assessment of the effect of one of the yeast recombination pathway genes on both, genome–wide YAC clone stability and the frequency of chimaeric clones. Expand
Primary structure of the chromosomal protein MC1 from the archaebacterium Methanosarcina sp. CHTI 55.
The covalent structure of protein MC1 and its effect on transcription of DNA in vitro is determined from automated sequence analysis of the protein and from structural data provided by peptides derived from cleavage of theprotein at aspartic acid and arginine residues. Expand
Primary structure of the chromosomal protein HMb from the archaebacteria Methanosarcina barkeri.
The amino acid sequence of the protein HMb, a protein of 93 residues (Mr 10757) which represents the major acid-soluble component of the Methanosarcina barkeri nucleoprotein complex, has beenExpand
Determination of the DNA-interacting region of the archaebacterial chromosomal protein MC1. Photocrosslinks with 5-bromouracil-substituted DNA.
Using photochemical crosslinking on 5-bromouracil-substituted DNA, the region of the protein that interacts with it is identified and the C-terminal part of the polypeptide chain, and the crosslinked amino-acids are in the region 74-86. Expand
Two regulatory elements of similar structure and placed in tandem account for the repressive activity of the first intron of the human apolipoprotein A-II gene.
It is shown that two sequence elements account for the repressive activity of this intron and correspond to negative regulatory elements termed NRE I and NRE II, which do not exhibit structural similarity with known NREs of other genes. Expand
Transcription of the human apolipoprotein A-II is down-regulated by the first intron of its gene.
The first intron of the human apoA-II gene down-regulates its own promoter and the ubiquitous thymidine kinase promoter both in HepG2 and Caco-2 cells and contains three sequences which bind nuclear proteins, thus demonstrating the presence of regulatory elements downstream of the transcription start site of the apOA- II gene. Expand