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In vivo hybridization of technetium-99m-labeled peptide nucleic acid (PNA).
The ability of the PNA to hybridize in vitro with its complement appeared to be unimpaired after conjugation and radiolabeling and 99mTc-PNA displays stability and pharmacokinetic properties suitable for eventual use as radiopharmaceuticals. Expand
Preparation and use of NHS-MAG3 for technetium-99m labeling of DNA.
It is concluded that the S-acetyl NHS MAG3 bifunctional chelator may prove to be an attractive alternative method of radiolabeling DNA and other biologically important molecules with 99mTc. Expand
Pretargeting with amplification using polymeric peptide nucleic acid.
It is concluded that amplification can be achieved in vivo using polymers of PNA followed by radiolabeled complementary PNA and that the application of pretargeting using polymer of P NA for amplification can improve localization. Expand
Pretargeting using peptide nucleic acid
Pretargeting studies in animals and humans have usually involved (strept)avidin and biotin. Depending on the particular strategy, endogenous biotin can adversely influence localization when theseExpand
Technetium-99m labeled epidermal growth factor-tumor imaging in mice.
We have shown previously that the epidermal growth factor peptide (EGF) may be radiolabeled with 99mTc at room temperature and neutral pH by using the N-hydroxysuccinimide ester of S-acetylExpand
Technetium-99m antibodies labeled with MAG3 and SHNH: an in vitro and animal in vivo comparison.
For two different antibodies and under the conditions of this study, the MAG3 chelator provided a 99mTc label with properties similar to that of SHNH moiety, with significant differences only in liver (Sandoz) and liver, spleen, intestines, stomach, and blood (C110). Expand
Labeling peptides with technetium-99m using a bifunctional chelator of a N-hydroxysuccinimide ester of mercaptoacetyltriglycine.
The use of NHS-S-acetyl-MAG3 may be a convenient method of radiolabeling peptides with 99mTc, and multiple peaks were present in the high-performance liquid chromatography radiochromatograms, especially of human neutrophil elastase inhibitor; however, most peaks could be shown to be labeled active peptide. Expand
NHS-MAS3: a bifunctional chelator alternative to NHS-MAG3.
It is concluded that labeling biocytin and amine-derivatized PNA with NHS-MAS3 compared to NHS-MAG3 provides simpler radiochromatographic profiles, improved stability of the label in serum and cysteine solution and can improve in vivo targeting. Expand
Early results in the irrational design of new bifunctional chelators
The development of a simple route for the synthesis of the N‐hydroxysuccinimide (NHS) ester of S‐acetyl‐protected mercaptoacetyltriglycine (MAG3) has opened the possibility of preparing novelExpand
The influence of temperature and alkaline pH on the labeling of free and conjugated MAG3 with technetium-99m.
S-acetyl MAG3 conjugated compounds may be radiolabeled at ambient temperature and neutral pH in most cases, and the radiochemical species produced appear to be identical to those formed when labeling is accomplished at 95 degrees C or pH 11. Expand