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Real-Time PCR Coupled with Automated DNA Extraction and Detection of Galactomannan Antigen in Serum by Enzyme-Linked Immunosorbent Assay for Diagnosis of Invasive Aspergillosis
TLDR
The LightCycler PCR assay could be used in association with the GM assay to improve the reliability of IA diagnosis and is retrospectively compared with an enzyme-linked immunosorbent assay for the detection of galactomannan in serum from 14 patients with IA. Expand
International Society of Human and Animal Mycology (ISHAM)-ITS reference DNA barcoding database--the quality controlled standard tool for routine identification of human and animal pathogenic fungi.
TLDR
An international consortium of medical mycology laboratories was formed aiming to establish a quality controlled ITS database, containing 2800 ITS sequences representing 421 fungal species, providing the medical community with a freely accessible tool to rapidly and reliably identify most agents of mycoses. Expand
Analysis of Microsatellite Markers ofCandida albicans Used for Rapid Typing
TLDR
Microsatellite marker analysis with multiplex PCR and automated procedures has a high throughput and should be suitable for large epidemiologic studies of C. albicans, confirming that candidemia usually originates from the colonizing isolate. Expand
Phagocytosis of Aspergillus fumigatus conidia by primary nasal epithelial cells in vitro
TLDR
In vitro model of primary cultures of human nasal epithelial cells (HNEC) can phagocytose fungal conidia but killing of phagcytosed conidia is low, although the spores do not germinate. Expand
Low prevalence of resistance to azoles in Aspergillus fumigatus in a French cohort of patients treated for haematological malignancies.
TLDR
The prevalence of azole resistance is currently low in the haematological population in the Paris area and surveillance programmes for azolesole resistance to adapt antifungal treatments are warranted for clinical isolates of A. fumigatus. Expand
Clinical Significance of Quantifying Pneumocystis jirovecii DNA by Using Real-Time PCR in Bronchoalveolar Lavage Fluid from Immunocompromised Patients
TLDR
A qPCR assay developed and compared the results with those of a routine immunofluorescence assay and clinical data, finding that Quantifying P. jirovecii DNA in BAL fluids independently of IFA positivity should be incorporated into the investigation of pneumonia in immunocompromised patients. Expand
Long-term outcome of AIDS-associated cryptococcosis in the era of combination antiretroviral therapy
TLDR
Overall survival after cryptococcosis has dramatically improved at the cART era and Immune restoration and low serum cryptococcal antigen titres are associated with lower cryptococcotic relapse rates. Expand
Microsatellite Marker Analysis as a Typing System for Candida glabrata
TLDR
Three polymorphic microsatellite markers, RPM2, MTI, and ERG3, were investigated using a multiplex PCR to obtain a rapid genotyping method for C. glabrata, and a factorial correspondence analysis did not indicate any correlation between the 21 multilocus genotypes and the clinical data. Expand
Diagnosis of invasive aspergillosis using bronchoalveolar lavage in haematology patients: influence of bronchoalveolar lavage human DNA content on real-time PCR performance
TLDR
Significantly higher β-globin levels were detected in Aspergillus PCR-positive than negative BAL fluids, suggesting that the β- globin level could reflect BAL yields and DNA extraction, and using β-Globin for the interpretation of fungal PCR could improve the negative predictive value of this test. Expand
Risks of ventilator-associated pneumonia and invasive pulmonary aspergillosis in patients with viral acute respiratory distress syndrome related or not to Coronavirus 19 disease
TLDR
A monocenter retrospective study comparing the incidence of VAP and invasive aspergillosis between patients with COVID-19-related acute respiratory distress syndrome (C-ARDS) and those with non-SARS-CoV-2 viral ARDS (NC-ARDS). Expand
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