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Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc). Expand
Pathway engineering for the production of aromatic compounds in Escherichia coli
It is shown that at least some of the PEP saved during glucose transport, can be redirected into the aromatic pathway, and this process should increase the availability of PEP for other biosynthetic reactions. Expand
Analysis of bacterial community during the fermentation of pulque, a traditional Mexican alcoholic beverage, using a polyphasic approach.
In this study, the characterization of the bacterial community present during the fermentation of pulque, a traditional Mexican alcoholic beverage from maguey (Agave), was determined for the firstExpand
Plasmids of Escherichia coli as cloning vectors.
The chapter discusses the properties of plasmid cloning vectors of E. coli as well as the techniques that can be used to select from a population of bacterial cells the ones carrying recombinant DNA molecules of interest. Expand
Cloning of the two pyruvate kinase isoenzyme structural genes from Escherichia coli: the relative roles of these enzymes in pyruvate biosynthesis
The cloning of the pykA and pykF genes from Escherichia coli, which code for the two pyruvate kinase isoenzymes, were reported, which were insertionally inactivated with antibiotic resistance markers and utilized to interrupt one or both pyk genes in the E. coli chromosome. Expand
Expression in Escherichia coli of chemically synthesized genes for human insulin.
Synthetic genes for human insulin A and B chains were cloned separately in plasmid pBR322 and fused to an Escherichia coli beta-galactosidase gene to provide efficient transcription and translation and a stable precursor protein. Expand
Peptides and genes coding for scorpion toxins that affect ion-channels.
The entire repertoire of toxins, independently of their specificity, was analyzed together by computational programs and a phylogenetic tree was built showing two separate branches, showing clear similarities among them. Expand
Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.
In vitro recombination via restriction endonucleases and the in vivo genetic translocation of the Ap resistance (Apr) gene resulted in the construction of a new cloning vehicle, the plasmid pBR313, which has a molecular weight of 5.8 Mdalton and has been characterized using thirteen restriction enzymes, six of which cleave the plasid at unique restriction sites. Expand
Metabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system
This study demonstrated for the first time the simultaneous inactivation of PTS and pykF as part of a strategy to improve SA production and its aromatic precursors in E. coli, with a resulting high yield of aromatic compounds on glucose of 0.5 mol/mol. Expand
Plasmid vector pBR322 and its special-purpose derivatives--a review.
A survey of derivative vectors based on the plasmid pBR322 to provide a survey of these derivative vectors and to summarize information currently available on pBR 322. Expand