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Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
In vitro recombination techniques were used to construct a new cloning vehicle, pBR322. This plasmid, derived from pBR313, is a relaxed replicating plasmid, does not produce and is sensitive toExpand
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Pathway engineering for the production of aromatic compounds in Escherichia coli
Glucose is the preferred substrate for certain fermentation processes. During its internalization and concomitant formation of glucose-6-phosphate through the glucose phosphotransferase system (PTS),Expand
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Analysis of bacterial community during the fermentation of pulque, a traditional Mexican alcoholic beverage, using a polyphasic approach.
In this study, the characterization of the bacterial community present during the fermentation of pulque, a traditional Mexican alcoholic beverage from maguey (Agave), was determined for the firstExpand
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Cloning of the two pyruvate kinase isoenzyme structural genes from Escherichia coli: the relative roles of these enzymes in pyruvate biosynthesis.
We report the cloning of the pykA and pykF genes from Escherichia coli, which code for the two pyruvate kinase isoenzymes (ATP:pyruvate 2-O-phosphotransferases; EC 2.7.1.40) in this microorganism.Expand
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Plasmids of Escherichia coli as cloning vectors.
Publisher Summary This chapter discusses the plasmids of Escherichia coli ( E. coli ) as cloning vectors. The essence of molecular cloning or recombination in vitro is the joining together in vitroExpand
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Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.
In vitro recombination via restriction endonucleases and the in vivo genetic translocation of the Ap resistance (Apr) gene resulted in the construction of a new cloning vehicle, the plasmid pBR313.Expand
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Expression in Escherichia coli of chemically synthesized genes for human insulin.
Synthetic genes for human insulin A and B chains were cloned separately in plasmid pBR322. The cloned synthetic genes were then fused to an Escherichia coli beta-galactosidase gene to provideExpand
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Metabolic engineering and protein directed evolution increase the yield of L‐phenylalanine synthesized from glucose in Escherichia coli
L‐phenylalanine (L‐Phe) is an aromatic amino acid with diverse commercial applications. Technologies for industrial microbial synthesis of L‐Phe using glucose as a starting raw material currentlyExpand
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Peptides and genes coding for scorpion toxins that affect ion-channels.
Most scorpion toxins are ligand peptides that recognize and bind to integral membrane proteins known as ion-channels. To date there are at least 202 distinct sequences described, obtained from 30Expand
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The role of penicillin amidases in nature and in industry.
Penicillin amidase (PA) is the enzyme used commercially for the production of semisynthetic penicillins. During the past decade, a detailed picture of the structure and regulation of the geneExpand
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