• Publications
  • Influence
Why highly expressed proteins evolve slowly.
Much recent work has explored molecular and population-genetic constraints on the rate of protein sequence evolution. The best predictor of evolutionary rate is expression level, for reasons thatExpand
  • 660
  • 58
Dynamic pattern formation in a vesicle-generating microfluidic device.
Spatiotemporal pattern formation occurs in a variety of nonequilibrium physical and chemical systems. Here we show that a microfluidic device designed to produce reverse micelles can generateExpand
  • 1,529
  • 52
A synthetic multicellular system for programmed pattern formation
Pattern formation is a hallmark of coordinated cell behaviour in both single and multicellular organisms. It typically involves cell–cell communication and intracellular signal processing. Here weExpand
  • 991
  • 34
Protein stability promotes evolvability.
The biophysical properties that enable proteins to so readily evolve to perform diverse biochemical tasks are largely unknown. Here, we show that a protein's capacity to evolve is enhanced by theExpand
  • 826
  • 28
Protein building blocks preserved by recombination
Borrowing concepts from the schema theory of genetic algorithms, we have developed a computational algorithm to identify the fragments of proteins, or schemas, that can be recombined withoutExpand
  • 315
  • 28
Molecular evolution by staggered extension process (StEP) in vitro recombination
We have developed a simple and efficient method for in vitro mutagenesis and recombination of polynu-cleotide sequences. The staggered extension process (StEP) consists of priming the templateExpand
  • 657
  • 22
Engineering microbial consortia: a new frontier in synthetic biology.
Microbial consortia are ubiquitous in nature and are implicated in processes of great importance to humans, from environmental remediation and wastewater treatment to assistance in food digestion.Expand
  • 612
  • 20
A microfabricated fluorescence-activated cell sorter
We have demonstrated a disposable microfabricated fluorescence-activated cell sorter (μFACS) for sorting various biological entities. Compared with conventional FACS machines, the μFACS providesExpand
  • 834
  • 19
Programmed population control by cell–cell communication and regulated killing
De novo engineering of gene circuits inside cells is extremely difficult, and efforts to realize predictable and robust performance must deal with noise in gene expression and variation in phenotypesExpand
  • 596
  • 17
Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli.
2-methylpropan-1-ol (isobutanol) is a leading candidate biofuel for the replacement or supplementation of current fossil fuels. Recent work has demonstrated glucose to isobutanol conversion through aExpand
  • 204
  • 16