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Stress-Dependent Regulation of FOXO Transcription Factors by the SIRT1 Deacetylase
The Sir2 deacetylase modulates organismal life-span in various species. However, the molecular mechanisms by which Sir2 increases longevity are largely unknown. We show that in mammalian cells, theExpand
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RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement
We have generated mice that carry a germline mutation in which a large portion of the RAG-2 coding region is deleted. Homozygous mutants are viable but fail to produce mature B or T lymphocytes. VeryExpand
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SIRT4 Inhibits Glutamate Dehydrogenase and Opposes the Effects of Calorie Restriction in Pancreatic β Cells
Sir2 is an NAD-dependent deacetylase that connects metabolism with longevity in yeast, flies, and worms. Mammals have seven Sir2 homologs (SIRT1-7). We show that SIRT4 is a mitochondrial enzyme thatExpand
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SIRT1 Regulates Circadian Clock Gene Expression through PER2 Deacetylation
The mammalian circadian timing system is composed of a central pacemaker in the suprachiasmatic nucleus of the brain that synchronizes countless subsidiary oscillators in peripheral tissues. TheExpand
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An early haematopoietic defect in mice lacking the transcription factor GATA-2
Blood cell development relies on the expansion and maintenance of haematopoietic stem and progenitor cells in the embryo. By gene targeting in mouse embryonic stem cells, we demonstrate that theExpand
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A role for the NAD-dependent deacetylase Sirt1 in the regulation of autophagy
We demonstrate a role for the NAD-dependent deacetylase Sirt1 in the regulation of autophagy. In particular, transient increased expression of Sirt1 is sufficient to stimulate basal rates ofExpand
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Efficient in vivo manipulation of mouse genomic sequences at the zygote stage.
We describe a transgenic mouse line carrying the cre transgene under the control of the adenovirus EIIa promoter that targets expression of the Cre recombinase to the early mouse embryo. To assessExpand
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Metabolic control of muscle mitochondrial function and fatty acid oxidation through SIRT1/PGC‐1α
In mammals, maintenance of energy and nutrient homeostasis during food deprivation is accomplished through an increase in mitochondrial fatty acid oxidation in peripheral tissues. An importantExpand
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Mammalian Sir2 Homolog SIRT3 Regulates Global Mitochondrial Lysine Acetylation
ABSTRACT Homologs of the Saccharomyces cerevisiae Sir2 protein, sirtuins, promote longevity in many organisms. Studies of the sirtuin SIRT3 have so far been limited to cell culture systems. Here, weExpand
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SIRT 3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation
Sirtuins are NAD-dependent protein deacetylases. They mediate adaptive responses to a variety of stresses, including calorie restriction and metabolic stress. Sirtuin 3 (SIRT3) is localized in theExpand
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