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Metabolic flux analysis of recombinant protein overproduction in Escherichia coli
TLDR
A metabolic model for cell growth and recombinant protein overproduction in Escherichia coli, which includes expression vector properties such as plasmid copy number and promoter strength as input parameters, was developed and was successfully simulated by metabolic flux analysis (MFA) computations. Expand
Integrated organic–aqueous biocatalysis and product recovery for quinaldine hydroxylation catalyzed by living recombinant Pseudomonas putida
TLDR
This study constitutes the first proof of concept on an integrated process for the oxyfunctionalization of toxic substrates with a water-incorporating hydroxylase and key parameters determining process performance were investigated and an efficient and easy method for product recovery was established. Expand
Regioselective aromatic hydroxylation of quinaldine by water using quinaldine 4-oxidase in recombinant Pseudomonas putida
TLDR
An alternative approach based on molybdenum (Mo)-containing dehydrogenases, which produce, rather than consume, reducing equivalents in the course of substrate hydroxylation and use water as the oxygen donor is discussed. Expand
Regio‐ and Stereoselective Hydroxylation
Anaerobic C-H oxyfunctionalization - coupling of nitrate reduction and quinoline hydroxylation in recombinant Pseudomonas putida.
TLDR
This study shows that molybdenum hydroxylase-based whole-cell biocatalysts enable completely anaerobic carbon oxyfunctionalization, when coupled to alternative respiration schemes such as nitrate respiration. Expand