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A physical map of the genome of buzura suppressaria singly-enveloped nuclear polyhedrosis virus (BsSNPV) was determined using six restriction endonucleases (RE), singly or in pairs, and Southern hybridizations of cloned or gel-purified DNA fragments. The maps of the genomes of BsSNPV and autographa californica multiply-enveloped nuclear polyhedrosis virus(More)
We studied in vivo binding sites of pemphigus foliaceus (PF) autoantibodies by immuno-gold labelling technique, and compared them with those of pemphigus vulgaris (PV). In early acantholytic lesions of PF, the bound antibodies indicated by 5 nm protein A-colloidal gold particles were observed on the surface of keratinocytes, with particular affinity for(More)
Mouse monoclonal antibodies to HTLV-1 core proteins, p19 and p24, were obtained by hybridoma technique. The specificity was studied by indirect immunofluorescence, enzyme-linked immunosorbent assay (ELISA), dot blot, and Western blot studies. Polyclonal antibodies reactive with HTLV-1 viral proteins were raised by rabbits and guinea pigs. A sandwich ELISA(More)
Cutaneous lesions of T-cell proliferative disorders are characterized by epidermotropic infiltration of the neoplastic cells and expression of intercellular adhesion molecule-1 (ICAM-1) and HLA-DR by lesional keratinocytes. Using cloned HTLV-1-infected T-cells obtained from patients with adult T-cell leukemia (ATL), we have studied immunobiological(More)
In order to understand the variety of HTLV-1-associated cutaneous diseases, we studied the cytological profile of HTLV-1-infected T-cell lines established from patients with adult T-cell leukaemia (ATL). Among four CD4+ cell lines, termed 16T(-), 35T(-), MH-1, and KS-2, the 16T(-) cells secreted elevated quantities of IL-4, IL-6 and IFN-gamma and expressed(More)
Localization of HTLV-I-associated antigens was studied in adult T cell leukemia (ATL) cells and HTLV-I-infected cell line cells using monoclonal and human polyclonal antibodies against the viral-related antigens. Two monoclonal antibodies that we obtained by hybridoma technique reacted with HTLV-I-virus core antigens, P19 and P24, respectively. Human(More)
A Gram-stain negative, strictly aerobic, non-motile, non-spore-forming, and rod-shaped bacterial strain designated FW-3T was isolated from fresh water and its taxonomic position was investigated by using a polyphasic approach. Strain FW-3T was found to grow at 10–37 °C and at pH 7.0 in the absence of NaCl on nutrient agar. On the basis of 16S rRNA gene(More)
Uncertainty is inherent in spatial data and spatio-temporal phenomena. Spatial data uncertainty generally refers to error, inexactness, fuzziness and ambiguity. The goals of research on spatial data uncertainty are to investigate how uncertainties arise, or are created and propagated in the spatial data process. Based on information theory, considering the(More)
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