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An electron microscopic, double-labelling immunocytochemical procedure was used to assess the level of fixed glutamate and glutamine in different cell profiles in ultrathin sections of rat cerebellar cortex. The procedure was based on sequential immunolabelling with two rabbit antisera, using gold particles of different sizes as markers and formaldehyde(More)
The distributions of five amino acids with well-established neuroexcitatory or neuroinhibitory properties were investigated in the feline vestibular complex. Consecutive semithin sections of plastic-embedded tissue were incubated with antisera raised against protein-glutaraldehyde conjugates of GABA, glycine, aspartate, glutamate and taurine. This approach(More)
Horseradish peroxidase (HRP) was injected or iontophoretically ejected in various thalamic nuclei in 63 adult cats. In 11 other animals HRP was deposited outside the thalamic territory. The number and distribution of labelled cells within the vestibular nuclear complex (VC) were mapped in each case. To a varying degree all subgroups of VC appear to(More)
The cerebellar afferents from the nucleus of the solitary tract were studied in cat by means of retrograde axonal transport of horseradish peroxidase. Labelled cells occurred bilaterally in the nucleus of the solitary tract following injections in various folia of the cerebellar vermis and in the flocculus (the positive cases are shown in Fig. 1A).(More)
A semiquantitative, electron microscopic immunocytochemical procedure based on the use of colloidal gold particles as markers was employed to analyze the subcellular distribution of glutamate and glutamine, a major glutamate precursor, in a subpopulation of spinocerebellar mossy fiber terminals. These terminals were identified by anterograde transport of a(More)
Details of cerebellar afferent projections from the vestibular nuclei were investigated by the method of retrograde axonal transport of horseradish peroxidase (HRP) in the cat. The distribution of labeled cells in the vestibular nuclei following HRP injections in various parts of the cerebellum indicates that vestibular neurons in the medial and descending(More)
Uptake and transport of horseradish peroxidase-wheat germ agglutinin conjugate (HRP-WGA) in intact and damaged passing fibers were studied by injections of the medulla and pons in 11 cats. Injections with evidence of damage to olivocerebellar fibers and cranial nerve fibers invariably lead to retrograde labeling of neurons in the inferior olive and cranial(More)
The cerebellar corticovestibular projection in the cat was studied by means of retrograde transport of HRP. After injections confined to the vestibular nuclei retrogradely labelled Purkinje cells were found ipsilaterally in vermal lobules I through X, crus I, the ventral paraflocculus and flocculus. The neurons projecting to the vestibular nuclei are(More)
Following injections of horseradish peroxidase (HRP) in the cerebellar cortex and nuclei of the cat, the distribution of labeled cells in the raphe nuclei was mapped. The findings confirm those made previously in studies of retrograde cell degeneration following cerebellar ablations (Brodal et al., 1960a), and in addition reveal new details in the(More)
A crossed projection from the red nucleus to the cerebellar cortex in the cat has been demonstrated by means of retrograde transport of the wheat germ agglutinin-horseradish peroxidase complex. The majority of projecting neurons are situated in the rostral part of the red nucleus and have a maximum diameter of more than 20 microns. The findings indicate(More)