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In the present study, we examined the in vitro regulation of 20-day-old rat Sertoli cell transferrin expression by tumor necrosis factor alpha (TNFalpha), a paracrine factor produced by germ cells. Addition of TNFalpha to highly purified cultured Sertoli cells resulted in a dose and time-dependent enhancement in the levels of transferrin mRNA(More)
Sertoli cells secrete plasminogen activators (PAs) on both sides of the blood-testis barrier, i.e., in the basal and apical compartments of the seminiferous tubules, whereas peritubular cells secrete plasminogen activator inhibitor-1 (PAI-1), a fast-acting and specific PA inhibitor. While it is likely that PAI-1 produced by peritubular cells counteracts the(More)
Previous studies suggested indirectly that residual bodies (RB) may play a role in the regulation of the proteolytic system within the testis and in the coordination of the spermatogenetic process. In the present study, we examined the effects of RB recovered from adult rat testes by centrifugal elutriation on Sertoli cell plasminogen activator (PA) levels,(More)
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