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Polypyrimidine tract binding protein (PTB) is an RNA-binding protein that regulates splicing by repressing specific splicing events. It also has roles in 3'-end processing, internal initiation of translation, and RNA localization. PTB exists in three alternatively spliced isoforms, PTB1, PTB2, and PTB4, which differ by the insertion of 19 or 26 amino acids,(More)
PTB (polypyrimidine tract-binding protein) is a repressive regulator of alternative splicing. We have investigated the role of PTB in three model alternative splicing systems. In the alpha-actinin gene, PTB represses the SM (smooth muscle) exon by binding to key sites in the polypyrimidine tract. Repressive binding to these sites is assisted by co-operative(More)
Jacky Chung, Joanne Lau, Lynn S. Cheng, R. Ian Grant, Fiona Robinson, Troy Ketela, Patricia P. Reis, Olga Roche, Suzanne Kamel-Reid, Jason Moffat, Michael Ohh, Bayardo Perez-Ordonez, David R. Kaplan & Meredith S. Irwin 1Department of Molecular Genetics, University of Toronto, 2Cell Biology Program, Hospital for Sick Children Research Institute, 3Department(More)
1 Auffarth GU, Wang L, Volcker HE. Keratoconus evaluation using the orbscan topography system. J Cataract Refract Surg 2000; 26: 222–228. 2 Cameron JA, Mahmood MA. Superior corneal thinning with pellucid marginal corneal degeneration. Am J Ophthalmol 1990; 109: 486–487. 3 Taglia DP, Sugar J. Superior pellucid marginal corneal degeneration with hydrops. Arch(More)
The p53 family of transcription factors is a key regulator of cell proliferation and death. In this report we identify the eukaryotic translation elongation factor 1-alpha 1 (eEF1A1) to be a novel p53 and p73 interacting protein. Previous studies have demonstrated that eEF1A1 has translation-independent roles in cancer. We report that overexpression of(More)
ΔNp63α is a critical pro-survival protein overexpressed in 80% of head and neck squamous cell carcinomas (HNSCCs) where it inhibits TAp73β transcription of p53-family target genes, which is thought to increase HNSCC resistance to chemotherapy-induced cell death. However, the mechanisms governing ΔNp63α function are largely unknown. In this study, we(More)
p73 encodes multiple functionally distinct isoforms. Proapoptotic TAp73 isoforms contain a transactivation (TA) domain, and like p53, have tumor suppressor properties and are activated by chemotherapies to induce cell death. In contrast, antiapoptotic ΔNp73 isoforms lack the TA domain and are dominant-negative inhibitors of p53 and TAp73. ΔNp73 proteins are(More)
Polypyrimidine tract-binding protein (PTB) is an hnRNP with four RRM type domains. It plays roles as a repressive alternative splicing regulator of multilple target genes, as well as being involved in pre-mRNA 3' end processing, mRNA localization, stability, and internal ribosome entry site-mediated translation. Here we have used a tethered function assay,(More)
5. Dresner S, Codere F, Browstein S, Jouve P. Lacrimal drainage system inflammatory masses from retained silicone tubing. Am J Ophthalmol 1984;98:609. 6. McCormick SA, Lindberg JV. Pathology of nasolacrimal duct obstruction. In: Lindberg JV, editor. Lacrimal surgery. London: Churchill Livingstone, 1988:169. 7. Welham RAN, Henderson PH. Results of(More)
The mitogen-activated protein kinase kinase kinases of the mixed-lineage kinase (MLK) family have been shown to activate the c-Jun N-terminal kinase (JNK) mitogen-activated protein kinase (MAPK) pathway, and to regulate the other two principal MAPK cascades, p38 and extracellular signal-regulated kinase (ERK). Although there is growing evidence for their(More)