F N Zolotarev

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The possibility of genetic identification of mutations in asporogenic yeast by the technique of intrageneric fusion of yeast protoplasts of Candida tropicals and Saccharomyces cerevisiae has been demonstrated for Candida tropicals strains G5-9 (Ade- Leu-) and G32-4 (Leu-). The mutations to auxotrophy ade- in the strain G5-9 and leu- in G32-4 of Candida(More)
Simultaneous circulation of different subtypes of influenza A viruses provides conditions for reassortant strains formation. A comparative investigation of genome of 47 influenza A virus strains (H1N1, H2N2, and H3N2) was carried out by competitive dot hybridization technique and sequence analysis of some of cDNA-copies of the virus genes. All the genes of(More)
A new method for genetic typing of influenza viruses using molecular hybridization of DNA-RNA was developed which consisted in addition to the hybridization solution, apart from the radioactively labeled probe, of RNA of a virus with known gene homologous to the plasmid DNA used as the probe but belonging to a different serosubtype of influenza A virus,(More)
The genotype of laboratory reassortants produced by co-cultivation of virulent A/USSR/2/85 (H3N2) virus and multi-passaged attenuation donor A/Leningrad/9/46 virus (H1N1) was determined by two different methods: a method of competitive dot hybridization (CDH) and polyacrylamide gel electrophoresis (PAGE) with virion RNAs urea. The results of the two methods(More)
The influenza A/Leningrad/134/47/57 (H2N2) (A/Len/47) cold-adapted virus expresses the ability to reproduce at 25 degrees C (the ca phenotype) and inability to reproduce at 40 degrees C (the ts phenotype). It was attenuated for mice. Reassortants of this donor virus with the genes coding for the surface glycoproteins from the epidemic viruses, i.e.(More)
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