F M Bartlett

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Prompted by concerns regarding outbreaks of food-borne illness which have occurred due to the consumption of commercial, nonpasteurized fruit juices contaminated with Escherichia coli O157:H7, the US Food and Drug Administration and Canadian Food Inspection Agency are considering several new safety standards to apply to fresh juices, including mandatory(More)
D-values (decimal reduction times) and z-values (increase in temperature required for a 1-log change in D-value) for Listeria monocytogenes Scott A were determined in liquid whole egg with nisin (0 or 10 microg ml(-1)) and NaCl (0 or 10%) by a submerged glass ampoule procedure. Samples were plated onto nonselective agar at appropriate intervals, and(More)
A total of 101 egg wash water samples from five different egg grading stations in eastern Ontario were analyzed for a variety of physical and chemical variables in an attempt to find a correlation with total bacterial counts. Temperature, pH, total chlorine, and percentage transmission at 600 nm (%T) were found to be significant variables, and a multiple(More)
A heat-stable extracellular protease from Pseudomonas fluorescens T16, a psychrotroph, was purified by affinity column chromatography on a carbobenzoxy-D-phenylalanine-triethylene tetramine-Sepharose-4B column. The purified enzyme is a monomer with a molecular weight of 38,905 +/- 2,000. In an analytical ultracentrifuge, the Schlieren profile revealed a(More)
A heat-stable extracellular protease from Pseudomonas fluorescens was purified by chromatography on a DEAE-cellulose column and gel filtration on a Sephadex G100 column. The homogeneous enzyme preparation was used to prepare antiserum in rabbits. The rabbit antiserum was used to study the antigenic relatedness of proteases from 19 psychrotrophic(More)
The viability of Listeria monocytogenes strains Scott A, 78-34, and 81-861 in artificial egg washwater at different temperatures and pH values was determined. After a 4-h incubation, less than a 1-log decrease in viability of strains Scott A and 78-34 was found at 33 degrees C with alkaline detergent (pH 8.0-10.5); however, up to a 3-log decrease in viable(More)
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