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Retroviral vectors are versatile gene transfer vehicles widely used in basic research and gene therapy. Mutation of retroviral integrase converts these vectors into transient, integration-deficient gene delivery vehicles associated with a high degree of biosafety. We explored the option to use integration-deficient retroviral vectors to achieve transient(More)
Human pluripotent stem cells (hPSCs) are defined by their indefinite self-renewal in vitro and their potential to differentiate into cells representative of all lineages of the mammalian gastrulation embryo. Several similarities link these genomically normal cells with features salient to malignancies, for example, when injected into immunodeficient mice,(More)
Correction of patient-specific induced pluripotent stem cells (iPSC) upon gene delivery through retroviral vectors offers new treatment perspectives for monogenetic diseases. Gene-modified iPSC clones can be screened for safe integration sites and differentiated into transplantable cells of interest. However, the current bottleneck is epigenetic vector(More)
Milk or a solution of gelatin and milk or commercial xanthine oxidase were lyophilized and the powdered freeze-dried materials crosslinked with glutaraldehyde. The resulting immobilized xanthine oxidase preparations have a good stability, are highly active and well suited for organic synthesis.
Cells of Arthrobacter X-4 were immobilized by entrapment in gelatin crosslinked with glutaraldehyde. The xanthine oxidase activity and stability were determined at various temperatures. In comparison with bovine milk xanthine oxidase the bacterial enzyme is more stable and has a different substrate specificity. 1-Methylxanthine was oxidized on a preparative(More)
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