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A procedure is described which allows the rapid permeabilization of yeast cells, Schizosaccharomyces pombe and Saccharomyces cerevisiae, for quantitative in situ assays of beta-galactosidase activity. Yeast cells are permeabilized by incubation in buffer containing 0.2% of the detergent sodium lauroyl sarcosinate without any need for washing or vortexing.(More)
An ultradian clock operates in fast growing cells of the large ciliate, Paramecium tetraurelia. The period of around 70 minutes is well temperature-compensated over the temperature range tested, i.e. between 18 degrees C and 33 degrees C. The Q10 between 18 degrees C and 27 degrees C is 1.08; above 27 degrees C there is a slight overcompensation. The(More)
Both a circadian clock and an ultradian clock (period 4-5 h) have previously been described for the ciliated protozoon Tetrahymena. The present communication demonstrates the existence of yet another cellular clock: an ultradian rhythm with a period of about 30 min. The period was found to be well temperature-compensated over the range studied, i.e.,(More)
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