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Energy transduction from proton gradients into ATP formation in chloroplast thylakoids has been hypothesized to be driven equally efficiently by localized domain delta mu H+ or by a delocalized delta mu H+ (Beard, W. A. and Dilley, R. A. (1988) J. Bioenerg. Biomembr. 20, 129-154). An important question is whether the apparent localized protonmotive force(More)
The involvement of ferric reduction in the iron uptake mechanism of iron-stressed Chlorella vulgaris from ferrioxamine B was investigated. Some comparative data for ferric-citrate was also obtained. EPR and a spectrophotometric assay were used to measure Fe(3+) reduction. These two methods differed in the absolute quantity but not in effectors of ferric(More)
Thylakoid membrane proteins are organized so as to shield 30-50 nmol H+ (mg Chl)-1 from freely equilibrating with either the external or the lumen aqueous phases. Amine groups provide binding sites for this metastable buffering array and can be quantitatively measured by acetylation using [3H]acetic anhydride. The principle of the assay is that a metastable(More)
Iron uptake from two Fe(3+)-hydroxamate siderophores, ferrioxamine B and Fe(3+)-rhodotorulate, by iron-stressed Chlorella vulgaris (ATCC strain 11468) was evaluated with some comparison to iron uptake from synthetic and organic acid ferric chelates. Iron-stress induced iron uptake from ferrioxamine B. Dissipation of the electrochemical gradient, via(More)
BACKGROUND Phycobiliproteins play an important role in fluorescent labeling, particularly for flow cytometry. The spectral properties of R-phycoerythrin (R-PE) and allophycocyanin (APC) have made them the dominant reagents in this class of fluorochromes. In this study, we evaluate a lesser-known but potentially important series of low-molecular weight(More)
Phycobilisomes are cyanobacterial photosynthetic energy transfer complexes partly composed of phycobiliproteins, proteins that are widely used as conjugable fluorochromes for flow cytometry. The brightness and photostability of phycobiliproteins suggest that intact phycobilisomes could constitute even brighter probes for fluorescence-based detection(More)
An easy, sensitive and direct fluorescent immunodetection method for proteins is described using the new fluorochrome PBXL-1 imaged with the FMBIO II Laser Scanning Imaging System. PBXL-1 is derived from a protein supra-molecular complex that contains a large number of chromophores. This complex, the phycobilisome, is extracted from a red alga then(More)
Electron paramagnetic resonance has been used to quantitate reduction of ferric ion bound to hydroxamate siderophores by iron-stressed fungi (DJ Ecker et al. 1982 J Biol Chem 257:8623-8626) and by iron-stressed Chlorella vulgaris (ATCC 11468) (FCT Allnutt, WD Bonner 1984 J Plant Nutr 7: 427-435). Application of this technique to quantitation of bound ferric(More)
A simple method for prompt fluorescent detection of inhibitors of human chorionic gonadotropin (hCG) binding to the extracellular domain of the human luteinizing hormone/chorionic gonadotropin (hLH/CG) receptor was developed for high throughput screening (HTS). Construction and analysis of a recombinant phage that displays the extracellular binding domain(More)
observed at concentrations exceeding their therapeutic ranges. A survey of library mass spectra indicated that some compounds can yield a 153 m/z or 167 m/z ion on electron impact ionization. These included allobarbital, p-aminosalicylic acid, and diphenylpyraline; however, these compounds were considered highly unlikely components of clinical specimens.(More)
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