Evgeny E Nikolsky

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Acetylcholinesterase (AChE) is an enzyme that hydrolyses the neurotransmitter acetylcholine, thereby limiting spillover and duration of action. This study demonstrates the existence of an endogenous mechanism for the regulation of synaptic AChE activity. At the rat extensor digitorum longus neuromuscular junction, activation of N-methyl-d-aspartate (NMDA)(More)
The kinetics of the phasic synchronous and delayed asynchronous release of acetylcholine quanta was studied at the neuromuscular junctions of aging rats from infant to mature animals at various frequencies of rhythmic stimulation of the motor nerve. We found that in infants 6 (P6) and 10 (P10) days after birth a strongly asynchronous phase of quantal(More)
In this study we demonstrate expression of the N-methyl-D-aspartate receptor NR1 subunit in the rat neuromuscular junction of skeletal muscles of different functional types (extensor digitorum longus, soleus, and diaphragm muscles) using fluorescence immunocytochemistry. Electron microscopic immunocytochemistry has shown that the NR1 subunit is localized(More)
The effects of high-frequency nerve stimulation (10-100 Hz) on the kinetics of evoked acetylcholine quanta secretion from frog motor nerve endings were studied. The amplitude and temporal parameters of uni- and multiquantal endplate currents were analysed to estimate the possible changes in the degree of synchrony of quantal release. The frog neuromuscular(More)
4 In the space flight environment and in models of the effects of weightlessness on Earth, the functional unloading of skeletal muscles leads to the development of the soocalled hypogravity syndrome expressed, in particular, in a number of morphological and funcc tional changes in the neuromuscular system. The characteristic features of this syndrome(More)
334 The cytoskeleton carries out many functions in cells of the nervous system, including synaptic formaa tion and function. Actin microfilaments, microtuu bules (MTs), and intermediate filaments are the main components of the cytoskeleton of motor axon termii nals. Microfilaments form a network and are often linked to the presynaptic membrane and its(More)
Experiments on frog neuromuscular junctions have demonstrated that asynchrony of the acetylcholine quantal release forming the multi-quantal evoked response at high-frequency synaptic activity is caused, in particular, by a decrease in velocity of the action potential propagation along the non-myelinated nerve endings, which is mediated by activation of the(More)
There is some evidence that glutamate (Glu) acts as a signaling molecule at vertebrate neuromuscular junctions where acetylcholine (ACh) serves as a neurotransmitter. In this study, performed on the cutaneous pectoris muscle of the frog Rana ridibunda, Glu receptor mechanisms that modulate ACh release processes were analyzed. Electrophysiological(More)
Gamma-aminobutyric acid (GABA) is an amino acid which acts as a neurotransmitter in the central nervous system. Here, we studied the effects of GABA on non-quantal, spontaneous, and evoked quantal acetylcholine (ACh) release from motor nerve endings. We found that while the application of 10 μM of GABA had no effect on spontaneous quantal ACh release, as(More)
5 We have earlier demonstrated that in the course of highhfrequency stimulation of the motor nerve changes in amplitude of the endplate successive potentials (currents) are not only related to variation of the quantal content of synaptic responses, but also result from asynchronous release of acetylcholine (ACh) in individual active zones [1]. Since(More)